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G8529

Sigma-Aldrich

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

recombinant, expressed in E. coli, lyophilized powder, ≥550 units/mg protein (biuret)

Synonym(s):

G-6-P-DH

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About This Item

CAS Number:
9001-40-5
Enzyme Commission number:
1.1.1.49 (BRENDA, IUBMB)
EC Number:
232-602-6
MDL number:
MFCD00081656
UNSPSC Code:
12352204
NACRES:
NA.54

recombinant

expressed in E. coli

Quality Level

300

form

lyophilized powder

specific activity

≥550 units/mg protein (biuret)

composition

Protein, 10-40% biuret

application(s)

agriculture

foreign activity

creatine phosphokinase, glutathione reductase, myokinase, NADH oxidase, NADPH oxidase, phosphoglucomutase, 6-phosphogluconic dehydrogenase, phosphoglucose isomerase, lactic dehydrogenase, hexokinase ≤0.01%

storage temp.

2-8°C

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Application

Glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides has been used:

  • in the coupled spectrophotometric assay for PGI (F6P to G6P reaction)
  • to study the starch time course using Nicotiana tabacum leaves
  • to determine the glucose content in the root and stem samples of Quercus velutina Lam. saplings
  • to assay glucose using a starch sample of wild-type and transgenic Arabidopsis whole seedlings

Biochem/physiol Actions

Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.
Glucose-6-phosphate dehydrogenase (G6PD) is expressed in all tissues and produces the reduced form of nicotinamide adenine dinucleotide phosphate (NADPH), which aids the cells to prevent oxidative stress. Insufficiency of G6PD is an X-linked, hereditary genetic defect. It is caused due to a common mutation in the G6PD gene.

Unit Definition

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.

Physical form

Lyophilized powder containing Ficoll and Tris buffer salts

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000406497
0000367009
0000367010
0000367011
0000367012

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. For Product No. G8529, Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides, can the Ficoll present in the product be removed?

    Ficoll is added to the enzyme preparation as a stabilizer. The enzyme may become unstable if the Ficoll is removed (i.e., through a dialysis step).

  6. In which solvent can Product No. G8529, Glucose- 6-phosphate Dehydrogenase from Leuconostoc mesenteroides, be dissolved?

    The product can be dissolved at 1 mg/ml in 5 mM glycine buffer, pH 8, (with or without 0.1% BSA) or in deionized water at 1 mg/ml (buffer is preferable)

  7. Can solutions of Product No. G8529, Glucose-6-phosphate Dehydrogenase, recombinant from Leuconostoc mesenteroides, be stored?

    Sigma has not evaluated the solution stability of this enzyme.  Perhaps solutions in buffer may be aliquotted and stored frozen at 20°C for about 1-2 months.  Sigma offers the enzyme in an ammonium sulfate suspension, product G8404, for solution storage at 2-8°C.

  8. What are the differences between Product No. G8529, Glucose-6-phosphate Dehydrogenase and Product No. G5885, Glucose-6-phosphate Dehydrogenase?

    Product No. G8529 is a recombinant protein from Leuconostoc mesenteroides, expressed in Escherichia coli.  Product No. G5885 is a non-recombinant protein from Leuconostoc mesenteroides.

  9. What is the molecular weight of Product No. G8529, Glucose-6-phosphate Dehydrogenase?

    Sigma has not determined the molecular weight of this protein, but a value of 55,000 daltons has been reported for the monomer molecular weight (the enzyme from Leuconostoc mesenteroides is reportedly a dimer protein) (Biochem. Biophys. Res. Comm. 59, 894 (1974)).

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Alyssa L Preiser et al.
Frontiers in plant science, 11, 580726-580726 (2020-12-29)
Phosphoglucoisomerase (PGI) isomerizes fructose 6-phosphate (F6P) and glucose 6-phosphate (G6P) in starch and sucrose biosynthesis. Both plastidic and cytosolic isoforms are found in plant leaves. Using recombinant enzymes and isolated chloroplasts, we have characterized the plastidic and cytosolic isoforms of
Benjamin J Reisman et al.
Nature chemical biology, 18(4), 360-367 (2021-12-04)
Cancer cells have long been recognized to exhibit unique bioenergetic requirements. The apoptolidin family of glycomacrolides are distinguished by their selective cytotoxicity towards oncogene-transformed cells, yet their molecular mechanism remains uncertain. We used photoaffinity analogs of the apoptolidins to identify
Shinichi Harada et al.
The Journal of pharmacology and experimental therapeutics, 344(1), 276-285 (2012-11-03)
Orexin-A (a glucose-sensing neuropeptide in the hypothalamus) and brain-derived neurotrophic factor (BDNF; a member of the neurotrophin family) play roles in many physiologic functions, including regulation of glucose metabolism. We previously showed that the development of postischemic glucose intolerance is
Myriam Ferrara et al.
Extremophiles : life under extreme conditions, 17(1), 53-62 (2012-11-03)
Psychrophilic organisms have evolved a number of modifications of cellular structures to survive in the cold environment; among them it is worth noting an increased efficiency of enzymes at lower temperatures. Glucose-6-phosphate dehydrogenase (G6PDH; EC 1.1.1.49) was purified and characterised
A O Abolaji et al.
Human & experimental toxicology, 32(2), 206-215 (2012-11-17)
Among the artemisinin-based combination therapy (ACT) regimens, artemisinin derivative, artemether in combination with lumefantrine (artemether-lumefantrine, AL) has achieved excellent results in the fight against malarial scourge. In this study, we evaluated the toxic potential of these drugs at the therapeutic
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Protocols

Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)

To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.

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