G6148
Glasgow Minimum Essential Medium
With L-glutamine, without tryptose phosphate broth and sodium bicarbonate, powder, suitable for cell culture
Synonym(s):
BHK medium, GMEM
Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
glucose: 4.5 g/L
phenol red: 0.0213 g/L
L-glutamine: 0.292 g/L
NaHCO3: no
shipped in
ambient
storage temp.
2-8°C
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General description
Glasgow Minimum Essential Medium is suitable for the study of genetic factors affecting cell competence.
Application
Glasgow Minimum Essential Medium has been used:
- in toxin entry assays
- for 3 H-palmitate labeling
- to culture embryonic stem cells
Preparation Note
Formulated to contain 12.5 grams of powder per liter of medium.
Supplement with 2.75 g/L sodium bicarbonate and Tryptose phosphate broth solution (T 8159) at 100 ml/L of medium.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
动植物来源培养基
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PloS one, 8(8), e71645-e71645 (2013-08-14)
Tcl1 is highly expressed in embryonic stem (ES) cells, but its expression rapidly decreases following differentiation. To assess Tcl1's roles in ES cells, we generated Tcl1-deficient and -overexpressing mouse ES cell lines. We found that Tcl1 was neither essential nor
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Naiara Clemente Tavares et al.
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Schistosomiasis is a neglected tropical disease (NTD) caused by helminthes from the Schistosoma genus. This NTD can cause systemic symptoms induced by the deposition of parasite eggs in the host liver, promoting severe complications. Functional studies to increase knowledge about
Tatsushi Miyazaki et al.
PloS one, 8(8), e71645-e71645 (2013-08-14)
Tcl1 is highly expressed in embryonic stem (ES) cells, but its expression rapidly decreases following differentiation. To assess Tcl1's roles in ES cells, we generated Tcl1-deficient and -overexpressing mouse ES cell lines. We found that Tcl1 was neither essential nor
Protocols
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性，应注意防潮。
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Instructions
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