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Merck
CN

G1166

Anti-GAD65 Antibody

mouse monoclonal, GAD-6

Synonym(s):

Anti-GAD 65

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD03455043

Key Documents

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Product Name

Monoclonal Anti-Glutamic Acid Decarboxylase 65 antibody produced in mouse, clone GAD-6, purified immunoglobulin, buffered aqueous solution

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

GAD-6, monoclonal

form

buffered aqueous solution

species reactivity

mammals (and some lower vertebrates)

technique(s)

immunohistochemistry: 1:100-1:500
western blot: 1:1,000-1:5,000

isotype

IgG2a

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... GAD2(2572)
mouse ... Gad2(14417)

Biochem/physiol Actions

Recognizes the lower molecular weight isoform of the two GAD isoforms identified in brain, GAD 65. Can be used for immunochemical localization of GABA secreting neurons.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

purified rat brain GAD 65.

Physical form

Solution in 0.01 M phosphate buffered saline, containing 0.04% sodium azide.

Preparation Note

Purified using Protein A chromatography.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
0000479510
0000479510
SLCK7532
SLBK7395V
061M1566

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Stathis S Leondopulos et al.
Journal of neural engineering, 9(2), 026015-026015 (2012-03-01)
Slow wave oscillations in the brain are essential for coordinated network activity but have not been shown to self-organize in vitro. Here, the development of dissociated hippocampal neurons into an active network with oscillations on multi-electrode arrays was evaluated in
Han-Byeol Kim et al.
Cell death discovery, 6, 73-73 (2020-08-21)
Neonatal maternal separation (NMS), as an early-life stress (ELS), is a risk factor to develop emotional disorders. However, the exact mechanisms remain to be defined. In the present study, we investigated the mechanisms involved in developing emotional disorders caused by
H Hu et al.
Neuroscience, 227, 211-222 (2012-10-09)
Cannabinoid 1 receptors (CB(1)) are highly expressed on presynaptic terminals in the brain where they are importantly involved in the control of neurotransmitter release. Alteration of CB(1) expression is associated with a variety of neurological and psychiatric disorders. There is
Nelyane N M Santana et al.
Frontiers in neuroanatomy, 12, 36-36 (2018-06-06)
In mammals, the suprachiasmatic nucleus (SCN) and the intergeniculate leaflet (IGL) are the main components of the circadian timing system. The SCN, classically known as the master circadian clock, generates rhythms and synchronizes them to environmental cues. The IGL is
Azadeh Yazdan-Shahmorad et al.
eLife, 7 (2018-05-29)
Brain stimulation modulates the excitability of neural circuits and drives neuroplasticity. While the local effects of stimulation have been an active area of investigation, the effects on large-scale networks remain largely unexplored. We studied stimulation-induced changes in network dynamics in
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Data Sheet - G1166

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