G0407
Granulocyte colony-stimulating factor human
≥95% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
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Product Name
Granulocyte colony-stimulating factor human, G-CSF, recombinant, expressed in E. coli, suitable for cell culture
biological source
human
Quality Level
recombinant
expressed in E. coli
Assay
≥95% (SDS-PAGE)
form
lyophilized powder
potency
0.01-0.1 ng/mL
quality
endotoxin tested
mol wt
18.8 kDa (175 amino acids including N-terminal methionine)
packaging
pkg of 5 and 25 μg
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This Item | AP307F | AP120F | AQ503F |
|---|---|---|---|
| species reactivity human | species reactivity rabbit | species reactivity human | species reactivity human |
| biological source goat | biological source goat | biological source goat | biological source goat |
| antibody form affinity purified immunoglobulin | antibody form affinity purified immunoglobulin | antibody form F(ab′)2 fragment of affinity isolated antibody | antibody form purified antibody |
| technique(s) immunofluorescence: suitable | technique(s) immunofluorescence: suitable | technique(s) immunofluorescence: suitable | technique(s) ELISA: suitable, immunofluorescence: suitable |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
General description
Application
Biochem/physiol Actions
Granulocyte colony-stimulating factor human (GCSF) interacts specifically with the immunoglobin (Ig) domain of the granulocyte colony-stimulating factor receptor (GCSFR).[2]
Granulocyte-colony stimulating factor (G-CSF) is a proliferation, differentiation, survival, and activation factor for hematopoietic cells of the restricted neutrophilic granulocyte lineage. It is produced by macrophages activated by endotoxin (LPS), by monocytes activated by TNF-α with IFNγ, by fibroblasts and endothelial cells activated by IL-1 or TNF-α, and by bone marrow stromal cells activated by IL-1 or LPS. It is suggested that during the inflammatory process endotoxins stimulate tissue macrophages to produce not only G-CSF but several other cytokines, including IL-1 and TNF-α, which in turn stimulate more G-CSF release from endothelial cells and fibroblasts. G-CSF can also synergize with IL-3 to shorten the G0 period of early hematopoietic progenitors. In addition to the namesake proliferative activity, G-CSF acts on mature neutrophils to enhance their survival and to stimulate their tumorcidal activity. Human G-CSF binds and activates a 130 kD to150 kD glycoprotein single chain receptor that has been classified as a member of the hematopoietic (cytokine) receptor family, the cytokine receptor class I, or the gp130 related cytokine receptor family (although it does not apparently bind to gp130). G-CSF receptors can be found on neutrophils, myeloid leukemia cells that respond to G-CSF, bone marrow cells of neutrophilic granulocyte lineage, and on placental trophoblasts, plus a soluble form may be expressed. Two forms of human G-CSF (177 and 174 amino acids) are synthesized from a single gene by alternative splicing, but murine G-CSF is a single expressed form of 178 amino acids. Human and murine G-CSF share 73% amino acid sequence homology and full cross-reactivity.
Physical form
Lyophilized from 0.2 μm-filtered solution of 10 mM HOAc containing 50 μg BSA per μg G-CSF
Analysis Note
The biological activity is measured in a cell proliferation assay using a murine myeloblastic cell line, NFS-60.
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Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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The clonogenic potential of selected CD34+ cells from patients with MDS appear preserved when tested ex vivo
Davison GM, et al.
Leukemia Research, 35, 1200-1204 (2011)
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The Journal of biological chemistry, 284(24), 16575-16583 (2009-04-14)
Numerous post-translational modifications have been identified in histones. Most of these occur within the histone tails, but a few have been identified within the histone core sequences. Histone core post-translational modifications have the potential to directly modulate nucleosome structure and
Eijiro Honjo et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 61(Pt 8), 788-790 (2006-03-03)
The granulocyte-colony stimulating factor (GCSF) receptor receives signals for regulating the maturation, proliferation and differentiation of the precursor cells of neutrophilic granulocytes. The signalling complex composed of two GCSFs (GCSF, 19 kDa) and two GCSF receptors (GCSFR, 34 kDa) consisting
Yukinori Okada et al.
Human molecular genetics, 19(10), 2079-2085 (2010-02-23)
Neutrophils are the most abundant subtype of white blood cells (WBCs). Although the regulation of the numbers of neutrophils would have substantial clinical impacts, the studies on the variations associated with neutrophil count had not been performed further. To investigate
Mohsen Esmaili et al.
Molecular biology research communications, 3(2), 141-147 (2014-06-01)
The present study aimed at investigating the beneficial effects of co-administering granulocyte colony-stimulating factor (G-CSF) and stem cell factor (SCF) in a model of chronic liver injury induced by thioacetamide (TAA). Biochemical and histopathology- cal examinations were performed on serum
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