G0407
Granulocyte colony-stimulating factor human
G-CSF, recombinant, expressed in E. coli, suitable for cell culture
Sign Into View Organizational & Contract Pricing
All Photos(1)
Recommended Products
biological source
human
Quality Level
recombinant
expressed in E. coli
Assay
≥95% (SDS-PAGE)
form
lyophilized powder
potency
0.01-0.1 ng/mL
quality
endotoxin tested
mol wt
18.8 kDa (175 amino acids including N-terminal methionine)
packaging
pkg of 5 and 25 μg
technique(s)
cell culture | mammalian: suitable
impurities
<1 EU/μg (LAL test)
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... CSF3(1440)
Looking for similar products? Visit Product Comparison Guide
Related Categories
General description
Granulocyte colony-stimulating factor human (GCSF) gene is mapped to human chromosome 17q21.1. Structurally, GCSF comprises a four α-helical bundle.
Application
Granulocyte colony-stimulating factor human has been used:
- in culturing human neutrophils
- as a growth factor component of double layer granulocyte-macrophage colony-forming unit (CFU-GM) culture system for CD34+ cells
- to test its protective effects chronic liver damage model
Biochem/physiol Actions
Granulocyte colony-stimulating factor human (GCSF) interacts specifically with the immunoglobin (Ig) domain of the granulocyte colony-stimulating factor receptor (GCSFR).
Granulocyte-colony stimulating factor (G-CSF) is a proliferation, differentiation, survival, and activation factor for hematopoietic cells of the restricted neutrophilic granulocyte lineage. It is produced by macrophages activated by endotoxin (LPS), by monocytes activated by TNF-α with IFNγ, by fibroblasts and endothelial cells activated by IL-1 or TNF-α, and by bone marrow stromal cells activated by IL-1 or LPS. It is suggested that during the inflammatory process endotoxins stimulate tissue macrophages to produce not only G-CSF but several other cytokines, including IL-1 and TNF-α, which in turn stimulate more G-CSF release from endothelial cells and fibroblasts. G-CSF can also synergize with IL-3 to shorten the G0 period of early hematopoietic progenitors. In addition to the namesake proliferative activity, G-CSF acts on mature neutrophils to enhance their survival and to stimulate their tumorcidal activity. Human G-CSF binds and activates a 130 kD to150 kD glycoprotein single chain receptor that has been classified as a member of the hematopoietic (cytokine) receptor family, the cytokine receptor class I, or the gp130 related cytokine receptor family (although it does not apparently bind to gp130). G-CSF receptors can be found on neutrophils, myeloid leukemia cells that respond to G-CSF, bone marrow cells of neutrophilic granulocyte lineage, and on placental trophoblasts, plus a soluble form may be expressed. Two forms of human G-CSF (177 and 174 amino acids) are synthesized from a single gene by alternative splicing, but murine G-CSF is a single expressed form of 178 amino acids. Human and murine G-CSF share 73% amino acid sequence homology and full cross-reactivity.
Physical form
Lyophilized from 0.2 μm-filtered solution of 10 mM HOAc containing 50 μg BSA per μg G-CSF
Analysis Note
The biological activity is measured in a cell proliferation assay using a murine myeloblastic cell line, NFS-60.
related product
Product No.
Description
Pricing
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
新产品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
The clonogenic potential of selected CD34+ cells from patients with MDS appear preserved when tested ex vivo
Davison GM, et al.
Leukemia Research, 35, 1200-1204 (2011)
Yukinori Okada et al.
Human molecular genetics, 19(10), 2079-2085 (2010-02-23)
Neutrophils are the most abundant subtype of white blood cells (WBCs). Although the regulation of the numbers of neutrophils would have substantial clinical impacts, the studies on the variations associated with neutrophil count had not been performed further. To investigate
Eijiro Honjo et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 61(Pt 8), 788-790 (2006-03-03)
The granulocyte-colony stimulating factor (GCSF) receptor receives signals for regulating the maturation, proliferation and differentiation of the precursor cells of neutrophilic granulocytes. The signalling complex composed of two GCSFs (GCSF, 19 kDa) and two GCSF receptors (GCSFR, 34 kDa) consisting
Mohsen Esmaili et al.
Molecular biology research communications, 3(2), 141-147 (2014-06-01)
The present study aimed at investigating the beneficial effects of co-administering granulocyte colony-stimulating factor (G-CSF) and stem cell factor (SCF) in a model of chronic liver injury induced by thioacetamide (TAA). Biochemical and histopathology- cal examinations were performed on serum
Rui Zhang et al.
Hematology (Amsterdam, Netherlands), 20(10), 555-560 (2015-06-13)
The treatment for acute myeloid leukemia (AML) remains an important clinical problem. Recently, hematopoietic stem cell therapy provides promising outcomes. In this study we examined the clinical effect of HLA-mismatched hematopoietic stem cell infusion combined with chemotherapy as a postremission
Articles
Read article on hematopoietic cytokines and hematopoiesis
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service