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F5392

Fibroblast Growth Factor-Basic from bovine pituitary

~90% (SDS-PAGE and N-terminal analysis), lyophilized powder, suitable for cell culture

Synonym(s):

FGF2, bFGF

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About This Item

CAS Number:
106096-93-9
UNSPSC Code:
12352202
NACRES:
NA.77
MDL number:
MFCD00081382

Key Documents

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Product Name

Fibroblast Growth Factor-Basic from bovine pituitary, FGF-Basic, suitable for cell culture

biological source

bovine pituitary glands

assay

~90% (SDS-PAGE and N-terminal analysis)

form

lyophilized powder

potency

0.03-3.0 ng/mL ED50/EC50

quality

endotoxin tested

mol wt

16.4 kDa

packaging

pkg of 1 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

<0.5 EU/vial

color

white

UniProt accession no.

P03969

storage temp.

−20°C

Quality Level

200

Gene Information

bovine ... FGF2(281161)

Analysis Note

The proliferative activity is tested in culture using fetal bovine heart endothelial cells.

Application

A natural mitogenic growth factor used in cell culture applications to study cell signaling and to promote fibroblast and endothelial cell growth and survival.
Fibroblast Growth Factor-Basic from bovine pituitary has been used:
  • In animal and human cell culture
  • In neuronal induction of adipose and bone marrow-derived rat mesenchymal stem cells
  • In the generation of directly reprogramming fibroblast
  • In human glioma cell culture

Biochem/physiol Actions

Fibroblast growth factor-2 (FGF2) stimulates cell proliferation, differentiation and migration. It is also known to participate in angiogenesis and carcinogenesis. During the estrous cycle, FGF2 expression is found to be maximum in porcine conceptuses and endometrium. Upregulation of this gene is also observed in pregnancy during peri-implantation. FGF2 is involved in embryogenesis, tissue repair and other metabolic pathways.

General description

The fibroblast growth factor-2 (FGF2) gene is predominantly expressed in uterus, cornea and vascular endothelial cells. The FGF2 gene is mapped to cattle chromosome 17.

Physical form

Lyophilized from a 0.2 μm filtered solution in sodium phosphate and sodium chloride containing 0.1 mg bovine serum albumin

Preparation Note

Purified glands by a modification of the method of Gospodarowicz, et al.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
SLBM9545V
040M6107
088K6123
037K6150
017K6102

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Devika Nurhasanah et al.
Asian Pacific journal of cancer prevention : APJCP, 24(1), 75-80 (2023-01-29)
Ficus septica is an Indonesian medicinal plant traditionally used to treat various illness, including cancer. The n-hexane insoluble fraction of the ethanolic extract of F. septica leaves (HIFFS) shows a potential anticancer activity against breast cancer cell line T47D. Considering
Random/aligned electrospun PCL/PCL-collagen nanofibrous membranes: comparison of neural differentiation of rat AdMSCs and BMSCs
Capkin M, et al.
Biomedical Materials (Bristol, England), 7(4), 045013-045013 (2012)
Single nucleotide polymorphism of FGF2 gene in Iranian Holstein proven bulls
Salehi A, et al.
Molecular Biology Research Communications, 4(1), 57-57 (2015)
Stimulatory effects of fibroblast growth factor 2 on proliferation and migration of uterine luminal epithelial cells during early pregnancy
Lim W, et al.
Biology of Reproduction, 96(1), 185-198 (2016)
Sensitivity of biochemical test in comparison with other methods for the detection of mycoplasma contamination in human and animal cell lines stored in the National Cell Bank of Iran
Kazemiha VM, et al.
Cytotechnology, 66(5), 861-873 (2014)
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