F3174
Fpg Protein from Escherichia coli
≥90% (SDS-PAGE), buffered aqueous glycerol solution, >20,000 units/mg protein, suitable for genomic analysis
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Synonym(s):
DNA-(apurinic or apyrimidinic site)lyase MutM (APlyase MutM), Fapy-DNAglycosylase, Formamidopyrimidine-DNA glycosylase, Fpg Protein from Escherichia coli, Recombinant, Fapy DNA glycosylase, Formamidopyrimidine DNA glycosylase, MutM
CAS Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.32
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biological source
Escherichia coli
Quality Level
recombinant
expressed in E. coli
Assay
≥90% (SDS-PAGE)
form
buffered aqueous glycerol solution
specific activity
>20,000 units/mg protein
mol wt
30.2 kDa (269 amino acids, predicted from the nucleotide sequence)
composition
protein, 0.1- 0.3 mg/mL Bradford
storage condition
(Tightly closed)
technique(s)
nucleic acid detection: suitable
UniProt accession no.
application(s)
genomic analysis
shipped in
wet ice
storage temp.
−20°C
Gene Information
Escherichia coli CFT073 ... mutM(1038243)
Escherichia coli K12 ... mutM(946765)
Related Categories
General description
Formamidopyrimidine-DNA glycosylase (Fpg) is a DNA repair enzyme found in Escherichia coli, which contains one zinc atom. Proximal to its C-terminal, it contains a zinc-finger motif of CC/CC type.
Fpg contains two domains separated by a flexible hinge.
Research area: Cell signaling
Research area: Cell signaling
Application
Fpg Protein from Escherichia coli has been used for the assessment of DNA oxidative damage using comet assay.
Biochem/physiol Actions
Formamidopyrimidine-DNA glycosylase (Fpg) cleaves double-stranded DNA containing the damaged base 8-oxo-7,8-dihydroguanine. It functions as an N-glycosylase and apurinic/apyrimidinic lyase.
Fpg is a key enzyme in the DNA base excision repair pathway (BER). It catalyses the excision of a broad spectrum of modified purines. Fpg has both DNA glycosylase activity that removes the mutated base and AP-lyase activity that releases ribose leaving both 5′- and 3′-phosphorylated ends in the DNA. The zinc finger motif at its C-terminus is responsible for the DNA binding and AP-lyase activity. In addition, its N-terminal proline acts as a nucleophile to produce a Schiff base intermediate that is essential for enzyme action.
Fpg specifically acts on 3′- and 5′-phosphodiester bonds.
Unit Definition
One unit will cleave 50% of 0.5 pmol of double-stranded DNA oligomer substrate (8-oxoguanine−mutated) in 10 min at 25 °C.
Physical form
Solution in 50% glycerol containing 50 mM potassium HEPES, pH 7.5, 1 mM DTT, 1 mM EDTA, and 200 mM NaCl.
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Lotte Frigaard Mandsberg et al.
FEMS microbiology letters, 324(1), 28-37 (2011-11-19)
Prevention and correction of oxidative DNA lesions in Pseudomonas aeruginosa is ensured by the DNA oxidative repair system (GO). Single inactivation of mutT, mutY and mutM involved in GO led to elevated mutation rates (MRs) that correlated to increased development
Yan Qi et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(4), 1086-1091 (2012-01-06)
Base excision repair of genotoxic nucleobase lesions in the genome is critically dependent upon the ability of DNA glycosylases to locate rare sites of damage embedded in a vast excess of undamaged DNA, using only thermal energy to fuel the
Therese Bergström et al.
Mutagenesis, 27(4), 511-517 (2012-04-03)
Vitamins with antioxidant properties have the ability to act as pro-oxidants, inducing oxidative damage and oxidative stress as opposed to preventing it. While vitamin supplements are commonly consumed, the scientific evidence for their health beneficial effects is inconclusive. In fact
Marlies Wallner et al.
Cancer prevention research (Philadelphia, Pa.), 6(10), 1056-1063 (2013-08-29)
The bile pigment bilirubin is a known antioxidant and is associated with protection from cancer and cardiovascular disease (CVD) when present in too strong concentrations. Unconjugated bilirubin (UCB) might also possess anti-genotoxic potential by preventing oxidative damage to DNA. Moderately
Lykke Forchhammer et al.
Mutagenesis, 27(6), 665-672 (2012-07-31)
There are substantial inter-laboratory variations in the levels of DNA damage measured by the comet assay. The aim of this study was to investigate whether adherence to a standard comet assay protocol would reduce inter-laboratory variation in reported values of
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