E1388
β-Endothelial Cell Growth Factor human
β-ECGF, recombinant, expressed in E. coli, suitable for cell culture
Synonym(s):
ECGS, β-ECGF
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About This Item
biological source
human
Quality Level
recombinant
expressed in E. coli
Assay
≥97% (SDS-PAGE)
form
lyophilized powder
potency
0.03-0.3 ng/mL ED50/EC50
quality
endotoxin tested
mol wt
predicted mol wt ~17 kDa
packaging
pkg of 25 μg
storage condition
avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
technique(s)
cell culture | mammalian: suitable
impurities
≤1.0 EU/μg protein Endotoxin level (LAL test)
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... VEGFB(7423)
Related Categories
General description
The β-endothelial cell growth factor (b-ECGF) gene encodes an angiogenic factor, and is mapped to human chromosome 11q13.1. Recombinant, human b-endothelial cell growth factor (b-ECGF) is a 14 amino acid N-terminal extension of acidic fibroblast growth factor. It is expressed in Escherichia coli. The 154 amino acid recombinant protein, generated after removal of the initiation methionine, has a predicted molecular mass of about 17 kDa.
Application
β-Endothelial Cell Growth Factor human has been used:
- in cell death assay
- in endothelial cell culture
- in human umbilical vascular endothelial cell (HUVEC) culture
Biochem/physiol Actions
β-Endothelial cell growth factor (β-ECGF) is a potent endothelial cell mitogen and its mitogenic activity is potentiated by heparin. β-ECGF stimulates the growth of quiescent HUVEC cells in culture. β-ECGF is associated with lipid and glucose metabolism. This growth factor induces neovascularization in disease conditions. Upregulation of β-ECGF is observed in diabetic retinopathy, and polycystic ovary syndrome. It is also associated with tumor proliferation and invasion. β-ECGF increases fatty acid transport proteins expression to improve endothelial mediated lipid transpor.
Other Notes
14 amino acid, N-terminal extended form of human FGF-acidic.
Physical form
Lyophilized from a 0.2 μm filtered solution in 10 mM MOPS, 50 mM ammonium sulfate, 0.2 mM DTT, and 0.2 mM EDTA, pH 7.2, containing 50 μg bovine serum albumin per 1 μg cytokine.
Analysis Note
The proliferative activity is tested in culture by using quiescent NR6R-3T3 fibroblasts.
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Plasma vascular endothelial growth factor B levels are increased in patients with newly diagnosed type 2 diabetes mellitus and associated with the first phase of glucose-stimulated insulin secretion function of beta-cell
Wu J, et al.
Journal of Endocrinological Investigation, 40(11), 1219-1226 (2017)
Autologous cell lines from circulating colon cancer cells captured from sequential liquid biopsies as model to study therapy-driven tumor changes
Soler A, et al.
Scientific reports, 8(1), 15931-15931 (2018)
Bioinformatics analyses of the role of vascular endothelial growth factor in patients with non-small cell lung cancer
Wang Y, et al.
PLoS ONE, 10(9), e0139285-e0139285 (2015)
Chuan-Rong Zhao et al.
Small (Weinheim an der Bergstrasse, Germany), 19(2), e2204694-e2204694 (2022-11-21)
Disturbed blood flow induces endothelial pro-inflammatory responses that promote atherogenesis. Nanoparticle-based therapeutics aimed at treating endothelial inflammation in vasculature where disturbed flow occurs may provide a promising avenue to prevent atherosclerosis. By using a vertical-step flow apparatus and a microfluidic
Eleanor G Lynam et al.
Methods in molecular biology (Clifton, N.J.), 2475, 223-228 (2022-04-23)
Endothelial cell proliferation rate is an important indicator of vascular health. Being able to detect the rate of endothelial cell proliferation, or cell cycle disturbances after intervention is a valuable tool for analysing any beneficial or detrimental effects of treatments
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