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Merck
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E0760

Endothelial cell growth supplement from bovine pituitary

Bioreagent, powder, suitable for cell culture

Synonym(s):

ECGS, Endothelial Mitogen

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.77

Key Documents

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Product Name

Endothelial cell growth supplement from bovine pituitary, ECGS, suitable for cell culture, BioReagent

biological source

bovine pituitary glands

product line

BioReagent

form

powder

potency

2-300 μg/mL ED50/EC50

quality

endotoxin tested

packaging

pkg of 15 mg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤20 EU/vial (LAL test)

color

brown to tan

solubility

water: soluble to hazy, red to red-brown (1 vial reconstituted with 1 ml 0.85% NaCl)

storage temp.

2-8°C

Quality Level

200

Application

Endothelial cell growth supplement from bovine pituitary has been used:
  • as a component in Roswell park memorial institute (RPMI) 1640 medium for endothelial cell culture
  • as a supplement in medium 199 for endothelial cell culture
  • as a supplement in Dulbecco′s modified eagle medium (DMEM):Ham′s F-12
  • as a supplement in Iscove′s modified Dulbecco′s medium (IMDM) for human umbilical vein endothelial cells (HUVEC) culture

Biochem/physiol Actions

Endothelial cell growth supplement (ECGS) has also been reported to be beneficial as a media supplement for the fusion and growth of hybridoma cells in monoclonal antibody production.

General description

Endothelial cell growth supplement (ECGS) is an extract of bovine pituitary glands containing growth promoting factors for vascular endothelial cells of mammalian origin. Endothelial cell growth supplement is prepared using a modification of a published method. It is lyophilized from a sterile solution containing NaCl and streptomycin sulfate.

pictograms

Health hazard
GHS08

signalword

Warning

hcodes

H361fd

Hazard Classifications

Repr. 2

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

监管及禁止进口产品
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Certificates of Analysis (COA)

Lot/Batch Number
SLCL4115
SLBS3583V
SLBL9789V
SLBC7104
091M6236

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In vitro biosynthesis of leukemia inhibitory factor/human interleukin for DA cells by human endothelial cells: differential regulation by interleukin-1 alpha and glucocorticoids
Grosset C, et al.
Blood, 86(10), 3763-3770 (1995)
Pei-Yu Chou et al.
Food science & nutrition, 8(2), 1093-1103 (2020-03-10)
This study investigated the effects of methanol extract Magnolia officinalis (MEMO) on baroreceptor reflex sensitivity (BRS) in the hypercholesterolemic rabbits and the involved molecular mechanisms. Male New Zealand white rabbits were randomly divided into Control (normal diet), Cholesterol (0.5% w/w
James F Dylewski et al.
MethodsX, 7, 101048-101048 (2020-09-19)
Glomerular endothelial cells (GEnC) are a specialized microvascular subset of endothelial cells that, when injured, result in many types of diseases within the kidney. Thus, techniques to study GEnC in a cell culture system are important to investigate mechanisms of
Autoantibodies against heat shock protein 60 mediate endothelial cytotoxicity.
Schett G, et al.
The Journal of Clinical Investigation, 96(6), 2569-2577 (1995)
Surface staining and cytotoxic activity of heat-shock protein 60 antibody in stressed aortic endothelial cells.
Xu Q, et al.
Circulation Research, 75(6), 1078-1085 (1994)
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Data Sheet - E0760

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