D9905
Denhardt′s Solution
for Northern and Southern blotting, lyophilized powder
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Synonym(s):
Denhardt′s Solution For Blotting Technique
UNSPSC Code:
12161700
NACRES:
NA.25
Recommended Products
grade
for molecular biology
Quality Level
form
lyophilized powder
technique(s)
Southern blotting: suitable
foreign activity
DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
storage temp.
2-8°C
General description
Denhardt′s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This powder can be used to make a 50x concentrate that can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.
Application
Denhardt′s Solution has been used:
- as a component in hybridization buffer for the hybridization of brain sections
- in in-situ hybridization peroxidase-(antiperoxidase method-whole mounts) (ISHPAP-WM) process
- in the preparation of hybridization mixture
Suitable as a blocking Buffer for Southern and Northern blot membranes.
Biochem/physiol Actions
Denhardt′s solution and gelatin-chrome alum can be used to treat chromosomes for G-banding with trypsin after in situ hybridization.
Components
Each vial of Denhardt′s powder contains 50mg Bovine Serum Albumin (BSA), 50mg Ficoll, and 50mg Polyvinylpyrrolidone (PVP).
Preparation Note
To make a 50x concentrate, dissolve entire contents of vial in 5 mL molecular biology grade water. Store at -20°C for up to two years.
related product
Product No.
Description
Pricing
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
含少量动物源组分生物产品
Certificates of Analysis (COA)
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Kunikowska G and Jenner P
Brain Research, 968(2), 206-218 (2003)
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
R E Kaye et al.
Proceedings of the National Academy of Sciences of the United States of America, 89(18), 8542-8546 (1992-09-15)
The inhibitory effects of cyclosporin A (CsA) and FK506 on Fc epsilon receptor type I-initiated increases in cytokine mRNA and the expression of their intracellular binding proteins were studied in interleukin 3 (IL-3)-dependent, mouse bone marrow-derived mast cells (BMMCs). In
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E J Mackie et al.
Journal of cell science, 103 ( Pt 3), 765-771 (1992-11-01)
The extracellular matrix glycoprotein, tenascin, is associated in vivo with mesenchyme undergoing osteogenesis and chondrogenesis, but is absent from mature bone and cartilage matrix. The expression of tenascin by osteoblastic cells in vitro has been investigated by immunoblotting and immunocytochemistry.
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