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D9893
pBR322 Plasmid DNA from E. coli RRI
lyophilized powder
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1 UNIT
CN¥4,469.43
5 UNITS
CN¥17,897.47
CN¥4,469.43
Available to ship onApril 27, 2025Details
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1 UNIT
CN¥4,469.43
5 UNITS
CN¥17,897.47
About This Item
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352200
eCl@ss:
32160414
NACRES:
NA.52
CN¥4,469.43
Available to ship onApril 27, 2025Details
New, lower price on this item!
Recommended Products
biological source
Escherichia coli
Quality Level
grade
Molecular Biology
form
lyophilized powder
mol wt
2.9 MDa
4363 bp
storage temp.
2-8°C
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Related Categories
1 of 4
This Item | D4904 | D3404 | D4154 |
|---|---|---|---|
| mol wt 2.9 MDa, 4363 bp | mol wt 2.9 MDa, 4363 bp | mol wt - | mol wt - |
| form lyophilized powder | form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| grade for molecular biology | grade for molecular biology | grade for molecular biology | grade for molecular biology |
| biological source Escherichia coli | biological source - | biological source - | biological source Escherichia coli (RRI) |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
General description
Plasmid pBR322 was one of the first multipurpose cloning vectors constructed for use in E. coli. This plasmid is derived from the ColE1-type plasmid pMB1 and shares the same type of replication mechanism and controls as ColE1 and relatives. Plasmid pBR322 confers resistance to ampicillin and tetracycline. The plasmid sequence has been published.
The plasmid has unique restriction sites within the gene for ampicillin resistance (Pst I, Pvu I, and Sca I), within the gene for tetracycline resistance (BamH I, BspM I, EcoR V, Nhe I, Nru I, Sal I, Sph I, and Xma III), and elsewhere (Aat II, Ava I, Bal I, Bsm I, BspM II, Cla I, EcoR I, Hind III, Nde I, Pvu II, Ssp I, Sty I, and Tth111 I).
The plasmid has unique restriction sites within the gene for ampicillin resistance (Pst I, Pvu I, and Sca I), within the gene for tetracycline resistance (BamH I, BspM I, EcoR V, Nhe I, Nru I, Sal I, Sph I, and Xma III), and elsewhere (Aat II, Ava I, Bal I, Bsm I, BspM II, Cla I, EcoR I, Hind III, Nde I, Pvu II, Ssp I, Sty I, and Tth111 I).
Specificity
Unique Sites: Within the gene for ampicillin resistance: Pst I, Pvu I, Sca I; Within the gene for tetracycline resistance: BamH I, BspM I, EcoR V, Nhe I, Nru I, Sal I, Sph I, Xma III; other unique sites: Aat II, Ava I, Bal I, Bsm I, BspM II, Cla I, EcoR I, Hind III, Nde I, Pvu II, Ssp I, Sty I, Tth111 I.
Application
Plasmid pBR322 was one of the first multipurpose cloning vectors constructed for use in Escherichia coli. This plasmid and derivatives have been used for a number of purposes including cloning, selection and expression of recombinant molecules, construction of shuttle vectors and vectors for nucleotide sequencing, studies of elements involved in gene expression, as plasmid DNA standards, and as a model system for studies on prokaryotic plasmid replication.
pBR322 Plasmid DNA from E. coli RRI has been used as a low molecular weight DNA to test the enzymatic activity of partially purified component from Perna viridis (Linn.)[1] It has also been used for investigating its effect on the calcium carbonate particle morphology by scanning electron microscopy studies[2]
Biochem/physiol Actions
One of the most commonly used cloning vectors, pBR322, confers resistance to ampicillin and tetracycline. The DNA sequence of the entire plasmid has been published.
This plasmid is derived from the ColE1-type plasmid pMB1 and shares the same type of replication mechanism and controls as ColE1 and relatives.
Components
DNA is provided as a powder, lyophilized from a solution of 1 mM Tris-HCl (pH 7.5) with 1mM NaCl and 1mM EDTA.
Other Notes
Accession number: J01749
Accession number: J01749
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Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Calcium carbonate microspheres with different surface structures were successfully prepared by the reaction of sodium carbonate with calcium chloride in the presence of deoxyribonucleic acid (DNA) at room temperature. The as-prepared products were characterized by scanning electron microscopy (SEM), transmission
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