Product Name
pBR322 DNA, solution, pkg of 200 μL (250 μg/ml)
form
solution
packaging
pkg of 200 μL (250 μg/ml)
manufacturer/tradename
Roche
storage condition
avoid repeated freeze/thaw cycles
storage temp.
−20°C (−15°C to −25°C)
Application
Select for recombinant DNA. The Pst I and Pvu I cleavage sites in the ampicillin gene, or the Cla I, Hind III, BamH I, and Sal I sites in the tetracycline gene allow the insertion of foreign DNA fragments, and inactivation of one of these genes. pBR322 DNA has been used in to study artificial metallonuclease activity.
General description
Plasmid for cloning of double-digested restriction fragments.
Other Notes
Chain Length 4,361 bp
For life science research only. Not for use in diagnostic procedures.
Physical form
Solution, 250 μg/ml, in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, ready-to-use
Preparation Note
Store at -15 to -25°C until use; once thawed, store at 2-8 °C.
Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
No data available
flash_point_c
No data available
Regulatory Information
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Andreea Prisecaru et al.
Journal of medicinal chemistry, 56(21), 8599-8615 (2013-10-18)
The synthetic chemical nuclease, [Cu(1,10-phenanthroline)2](2+), has stimulated research within metallonuclease development and in the area of cytotoxic metallodrug design. Our analysis reveals, however, that this agent is "promiscuous" as it binds both dsDNA and protein biomolecules, without specificity, and induces
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