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D8893

Sigma-Aldrich

Dexamethasone

powder, γ-irradiated, BioXtra, suitable for cell culture, ≥80% (HPLC)

Synonym(s):

(11β,16α)-9-Fluoro-11,17,21-trihydroxy-16-methylpregna-1,4-diene-3,20-dione, 9α-Fluoro-16α-methyl-11β,17α,21-trihydroxy-1,4-pregnadiene-3,20-dione, 9α-Fluoro-16α-methylprednisolone, Prednisolone F

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About This Item

Empirical Formula (Hill Notation):
C22H29FO5
CAS Number:
Molecular Weight:
392.46
Beilstein:
2066651
EC Number:
MDL number:
UNSPSC Code:
12352209
PubChem Substance ID:
NACRES:
NA.77

biological source

synthetic (organic)

Quality Level

sterility

γ-irradiated

product line

BioXtra

Assay

≥80% (HPLC)

form

powder

potency

4-500 ng/mL

technique(s)

cell culture | mammalian: suitable

mp

262-264 °C (lit.)

solubility

ethanol: 1 mL/vial, clear

shipped in

ambient

storage temp.

2-8°C

SMILES string

C[C@@H]1C[C@H]2[C@@H]3CCC4=CC(=O)C=C[C@]4(C)[C@@]3(F)[C@@H](O)C[C@]2(C)[C@@]1(O)C(=O)CO

InChI

1S/C22H29FO5/c1-12-8-16-15-5-4-13-9-14(25)6-7-19(13,2)21(15,23)17(26)10-20(16,3)22(12,28)18(27)11-24/h6-7,9,12,15-17,24,26,28H,4-5,8,10-11H2,1-3H3/t12-,15+,16+,17+,19+,20+,21+,22+/m1/s1

InChI key

UREBDLICKHMUKA-CXSFZGCWSA-N

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Application

Dexamethasone has been used as a component of osteogenic differentiation medium. It has also been used to study apoptosis, cell signaling pathways and gene expression.

Biochem/physiol Actions

Dexamethasone has immunosuppressant and antiemetic properties. It is used to treat postoperative nausea and vomiting (PONV) and chemotherapy-induced nausea and vomiting (CINV). Dexamethasone plays an important role in cardiac transplant, sepsis, cancer and other pathologic disorders. It elevates the level of heat shock proteins. It is an anti-inflammatory glucocorticoid with a range of effects on cell survival, cell signaling and gene expression.
Glucocorticoid anti-inflammatory agent. Regulates T cell survival, growth, and differentiation. Inhibits the induction of nitric oxide synthase.

Physical form

powder 2-8 °C; stock-frozen in working aliquots, avoid repeated freeze/thaw

Reconstitution

To prepare 20 μg/ml stock solution: add 1 ml absolute ethanol; gently swirl to dissolve; add 49 ml sterile medium while mixing.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Repr. 1B

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

监管及禁止进口产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the best way to dissolve Product D8893, Dexamethasone?

    The solubility of dexamethasone is <0.01 mg/mL in water and 24 mg/mL in H5784 (a 45% w/v solution of 2-hydroxypropyl-β-cyclodextrin).

  6. What is the solubility of Product D8893, Dexamethasone, in ethanol?

    The product is soluble in 95% ethanol (1mg/mL), yielding a clear, colorless solution. A 20 μg/mL stock solution can be formed by adding 49 mL of media to 1 mL of a 1 mg/mL ethanol stock.

  7. What is the solubility of Product D8893, Dexamethasone, in DMSO?

    The solubility of dexamethasone in DMSO is 606 mg/mL at 25°C.

  8. What solution stability information do you have available for Product D8893, Dexamethasone?

    We would recommend that you store stock solutions in working aliquots frozen at -20°C. It is best to avoid repeated freeze/thaw cycles.

  9. How do the dexamethasone products D8893 and the D4902 compare?

    They are the same except for the sterility. D8893 has been g-irradiated, so it is sterile. The D4902 is not necessarily sterile. Both are cell culture tested by Sigma.

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Acta Biomaterialia, 16(5), 35-48 (2015)
Pharmacology and Physiology for Anesthesia: Foundations and Clinical Application (2013)
Substrate elasticity regulates adipose-derived stromal cell differentiation towards osteogenesis and adipogenesis through $\beta$-catenin transduction
Xie J, et al.
Acta Biomaterialia, 79(5), 83-95 (2018)
C A Smith et al.
Journal of tissue engineering and regenerative medicine, 9(5), 595-604 (2014-06-20)
Fresh-frozen biological allograft remains the most effective substitute for the 'gold standard' autograft, sharing many of its osteogenic properties but, conversely, lacking viable osteogenic cells. Tissue engineering offers the opportunity to improve the osseointegration of this material through the addition

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