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D3687

Sigma-Aldrich

DirectLoad PCR 100 bp Low Ladder

ready-to-use marker for DNA electrophoresis

Synonym(s):

100bp ladder for gel electrophoresis, 100bp maker, DirectLoad marker, RTU DNA ladder, RTU DNA marker, agarose gel electrophoresis ladder, agarose gel electrophoresis marker

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4 L
CN¥2,156.63

CN¥2,156.63


Estimated to ship onOctober 17, 2025Details


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4 L
CN¥2,156.63

About This Item

UNSPSC Code:
41105335
NACRES:
NA.31

CN¥2,156.63


Estimated to ship onOctober 17, 2025Details


Request a Bulk Order

grade

Molecular Biology

Quality Level

form

liquid

usage

75 uses

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

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This Item
D6510027099732222
Quality Level

100

Quality Level

100, 200

Quality Level

200

Quality Level

200

technique(s)

HPLC: suitable

technique(s)

-

technique(s)

gas chromatography (GC): suitable

technique(s)

-

assay

≥99.9%

assay

≥99.5%

assay

≥99.8%

assay

≥99.9% (GC)

form

liquid

form

liquid

form

liquid

form

liquid

UV absorption

λ: 235 nm Amax: 1.00, λ: 250 nm Amax: 0.05, λ: 340-400 nm Amax: 0.01, λ: 240 nm Amax: 0.20, λ: 260 nm Amax: 0.02

UV absorption

-

UV absorption

-

UV absorption

-

vapor pressure

24.45 psi ( 55 °C), 6.83 psi ( 20 °C)

vapor pressure

24.45 psi ( 55 °C), 6.83 psi ( 20 °C)

vapor pressure

24.45 psi ( 55 °C), 6.83 psi ( 20 °C), 6.86 psi ( 20 °C)

vapor pressure

24.45 psi ( 55 °C), 6.83 psi ( 20 °C)

General description

Sigma′s DirectLoad PCR 100 bp Low Ladder contains 10 dsDNA fragments from 100 − 1,000 bases, evenly spaced in 100 bp repeats. Ladder should be loaded in a single lane on an agarose gel.

Application

Suitable for size determination of PCR generated DNA fragments with either agarose or polyacrylamide gels.

Features and Benefits

  • Ready-to-load
  • Easy-to-use
  • Popular band sizes

Components

Sigma′s DirectLoad PCR 100 bp Low Ladder is provided in a solution of 5% glycerol, 4.2 mM EDTA, 0.09% orange G, and 0.0125% xylene cyanol.

Other Notes

For optimal resolution, the recommended agarose gel concentration is 2.0%.

Legal Information

DirectLoad is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Articles

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

Protocols

Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

JumpStart™ Taq DNA Polymerase is an antibody-inactivated, hot start enzyme.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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