CS0980
Chitinase Assay Kit
sufficient for 100 multiwell tests
Synonym(s):
Chitinase Activity Detection Kit
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About This Item
Recommended Products
usage
sufficient for 100 multiwell tests
Quality Level
absorbance ratio
405 nm (Absorbance)
shipped in
wet ice
storage temp.
2-8°C
Gene Information
human ... CHIA(27159) , CHIT1(1118)
Related Categories
1 of 4
This Item | CLS3233 | CLS3232 | CLS3264 |
|---|---|---|---|
| sterility sterile | sterility sterile | sterility sterile | sterility sterile |
| material polystyrene , silicon tubing (6′′) | material polystyrene , silicon tubing (6′′) | material polystyrene , silicon tubing (6′′) | material square bottom flask |
| binding type Tissue Culture (TC)-treated surface | binding type - | binding type - | binding type Tissue Culture (TC)-treated surface |
| packaging bag of 1 ea, case of 2 ea | packaging bag of 1 ea, case of 1 ea | packaging bag of 1 ea, case of 1 ea | packaging case of 1 ea |
| surface area 8500 cm2 , cell growth area | surface area 8500 cm2 , cell growth area | surface area 21,250 cm2 , cell growth area | surface area - |
General description
Application
The kit provides all the reagents required for efficient detection of chitinase activity in fungal and bacterial growth media, macrophage lysates, and purified enzyme preparations. In addition, the kit provides three different substrates for the detection of the various types of the chitinolytic activity:
- 4-Nitrophenyl N,N′-diacetyl-β-D-chitobioside - substrate suitable for exochitinase activity detection (chitobiosidase activity)
- 4-Nitrophenyl N-acetyl-β-D-glucosaminide - substrate suitable for exochitinase activity detection (β-N-acetylglucosaminidase activity)
Biochem/physiol Actions
The chitinolytic enzymes are also categorized based on their enzymatic action on chitin substrates. Endochitinases are defined as the enzymes catalyzing the random cleavage at internal points in the chitin chain. Exochitinases catalyze the progressive release of acetylchitobiose or N-acetylglucosamine from the non-reducing end of chitin, and are referred to as chitobiosidase and β-N-acetylglucosaminidase, respectively.
Chitinases perform different functions in different organisms. In bacteria, they are mainly involved in nutritional processes. In yeast and various fungi, these enzymes participate in morphogenesis. In animals and plants, chitinases primarily play a role in the defense of the organism against pathogen attack.
Analysis Note
Kit Components Only
- Assay Buffer 20 mL
- 4-Nitrophenyl N-acetyl-β-D-glucosaminide 10 mg
- 4-Nitrophenyl N,N′-diacetyl-β-D-chitobioside 5 mg
- 4-Nitrophenyl β-D-N,N′N′′-triacetylchitotriose 1 mg
- Chitinase from Trichoderma viride 1 mg
- p-Nitrophenol Solution, 10 mM 1 mL
- Sodium Carbonate 1 g
related product
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Carc. 2 - Eye Irrit. 2 - Resp. Sens. 1 - STOT RE 2 Oral
Target Organs
Liver,Kidney
Storage Class Code
10 - Combustible liquids
Regulatory Information
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What has Product CS0980, Chitinase Assay Kit, been tested on?
This kit has been tested on Trichoderma viride, Streptomyces griseus and human macrophages.
What is the recommended substrate concentration for Product CS0980, Chitinase Assay Kit?
A 1mg/mL substrate solution is recommended. If required, the 4-Nitrophenyl-beta-D-N,N',N''-triacetylchitotriose and the 4-Nitrophenyl N,N'-diacetyl-beta-D-chitobioside can be used at lower concentrations in the reaction (0.5 or 0.2mg/mL respectively).
How do you make the substrate in Product CS0980, Chitinase Assay Kit, go into solution?
The substrates do not dissolve easily in the buffer. It may take ~1 hour of shaking to completely dissolve the substrates. Use of a larger container (15 mL) may aid dissolution.
What is the stability of the Product CS0980, Chitinase Assay Kit, substrate solution (s)?
The substrate solution(s) should be stored on ice during the experiment. For storage up to one month, freeze at -20°C or below.
How many reactions will each substrate in Product CS0980, Chitinase Assay Kit, provide?
If used at a concentration of 1mg/mL: * 4-Nitrophenyl N-acetyl-b-D-glucosaminide (Product No. N9376) -100 reactions; * 4-Nitrophenyl N,N’-diacetyl-b-D-chitobioside (Product No. N6133) -50 reactions; * 4-Nitrophenyl-b-D-N,N',N''-triacetylchitotriose (Product No. N8638) -10 reactions.
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