SAE0158
灰链霉菌来源几丁质酶
chromatographically purified, lyophilized powder, free of DNA contaminants, suitable for Microbiome research
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Chitinase from Streptomyces griseus, (1→4)-2-acetamido-2-deoxy-beta-D-glucan glycanohydrolase), Chitodextrinase, Hydrolitic enzyme, Lytic Enzyme, Poly(β-(1→4)-[2-acetamido-2-deoxy-D-glucoside]) glycanohydrolase, Poly(1,4-β-[2-acetamido-2-deoxy-D-glucoside]) glycanohydrolase, beta-1,4-poly-N-acetyl glucosamidinase, poly-beta-glucosaminidase
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一般描述
Chitinase is an extracellular complex of enzymes that degrade chitin. Chitin is a cell wall component of Fungi and exoskeletal essentials of different organisms which reshape their own chitin or digest/dissolve the chitin of other organisms (insects, fungi, yeast, and algae, and in the internal structures of other vertebrates). Chitinases have been detected in many microorganisms and in plants. In fungi, chitinases assist in morphogenesis, to break down the inherent chitin content of fungal cell walls. Plant chitinases help in resistance to fungal attack and counteracting fungal growth, by targeting those same fungal cell walls. In bacteria, bacterial chitinases assist in utilizing chitin as a carbon source and as an energy source. Streptomyces griseus produces multiple chitinases of different molecular masses after growth induction with chitin as the carbon source.
The enzymatic hydrolysis of chitin to N-acetyl-D-glucosamine involves two consecutive enzyme reactions:
The enzymatic hydrolysis of chitin to N-acetyl-D-glucosamine involves two consecutive enzyme reactions:
- The first reaction, chitodextrinase-chitinase, is a poly(β-(1→4)-[2-acetamido-2-deoxy-D-glucoside])- glycanohydrolase, which removes chitobiose units from chitin.
- The second activity is N-acetyl-glucosaminidasechitobiase, which cleaves the disaccharide to its monomer subunits, N-acetyl-D-glucosamine.
应用
- Agriculture fields: control pathogens.
- Human health care: Asthma.
- Pharma: preparation of chitooligosaccharides and N-acetyl D glucosamine,
- Preparation of single-cell protein
- Isolation of protoplasts from fungi and yeast
- Control of pathogenic fungi
- Treatment of chitinous waste, mosquito control and morphogenesis
特点和优势
This Chitinase is free of DNA contaminates, suitable for microbiome research. Cell walls digestion is performed safely by chitin degradation in fungi and other organisms.
单位定义
One unit will liberate 1.0 mg of N-acetyl-D-glucosamine from chitin per hour at pH 6.0 at 25 °C in a 2-hour assay.
Journal of molecular biology, 358(2), 472-484 (2006-03-07)
Chitinase C (ChiC) from Streptomyces griseus HUT6037 was the first glycoside hydrolase family 19 chitinase that was found in an organism other than higher plants. An N-terminal chitin-binding domain and a C-terminal catalytic domain connected by a linker peptide constitute
Journal of pharmacy & bioallied sciences, 5(1), 21-29 (2013-04-06)
Chitin, the second most abundant polysaccharide in nature after cellulose, is found in the exoskeleton of insects, fungi, yeast, and algae, and in the internal structures of other vertebrates. Chitinases are enzymes that degrade chitin. Chitinases contribute to the generation
Journal of bioscience and bioengineering, 89(1), 27-32 (2005-10-20)
A 49-kDa chitinase (pI7.3) was purified to homogeneity from the culture supernatant of Streptomyces griseus HUT 6037 by ultrafiltration, DEAE-Sephadex A-50 and Sephadex G-100 column chromatographies, and chromatofocusing. The purified enzyme was stable up to 40 degrees C. The N-terminal
商品
Enzymatic cell lysis and protoplast prep processes demystified, revealing complex enzymatic reactions during sample preparation.
裂解酶提供了一种非机械式细胞裂解和原生质体准备方法。这看上去是个非常简单的过程:只需加入酶,将试管放入水浴然即可。但这过程中到底发生了什么?
Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.
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