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Safety Information

CS0720

Sigma-Aldrich

Citrate Synthase Assay Kit

1 kit sufficient for 100 reactions (using a 1 ml cuvette), 1 kit sufficient for 480 reactions (using 96 multiwell plates)

Synonym(s):

Citrate Synthase Activity Test Kit

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25 EA
CN¥577.48

CN¥577.48

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25 EA
CN¥577.48

About This Item

UNSPSC Code:
12161503
NACRES:
NA.28

CN¥577.48

Please contact Customer Service for Availability
Request a Bulk Order

Quality Level

300

usage

 kit sufficient for 100 reactions (using a 1 ml cuvette)
 kit sufficient for 480 reactions (using 96 multiwell plates)

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CS(1431)

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HCVD3MAG67K

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HCVD2MAG67K

MILLIPLEX® Human Cardiovascular Disease (CVD) Magnetic Bead Panel 2 - Cardiovascular Disease Multiplex Assay

assay range

accuracy: 100%
(CK-MB), accuracy: 102%
(Oncostatin (OSM)), accuracy: 96%
(FABP3), accuracy: 97%
(NT proBNP), standard curve range: 2.7-2,000 pg/mL
(Placental Growth Factor (PlGF)), standard curve range: 6.9-5,000 pg/mL
(CXCL6, Oncostatin (OSM)), standard curve range: 685.9-500,000 pg/mL
(FABP4), inter-assay cv: <20%
intra-assay cv: <10%, accuracy: 103%
(CXCL6/GCP-2), standard curve range: 137.2-100,000 pg/mL
(FABP3), standard curve range: 68.6-50,000 pg/mL
(BNP, CK-MB, Troponin I (TnI)), accuracy: 102%
(LIGHT), standard curve range: 34.3-25,000 pg/mL
(NT proBNP, CXCL16, Endocan-1 (ESM-1), LIGHT), accuracy: 96%
(Placental Growth Factor (PlGF))

assay range

accuracy: 103%
(Thrombomodulin), accuracy: 104%
(Pentraxin-3 (PTX3)), accuracy: 104%
(Tissue Factor (TF)), accuracy: 106%
(Pecam-1), accuracy: 109%
(sE-Selectin), standard curve range: 0.01-5 ng/mL
(Troponin T (TnT)), standard curve range: 0.04-30 ng/mL
(Follistatin (FST)), standard curve range: 0.14-100 ng/mL
(Pentraxin-3 (PTX3) & Tissue Factor (TF)), standard curve range: 0.27-200 ng/mL
(Pecam-1), standard curve range: 0.34-250 ng/mL
(Thrombomodulin), standard curve range: 2.7-2,000 ng/mL
(sE-Selectin), inter-assay cv: <20%
intra-assay cv: <10%

assay range

accuracy: 102%
(Haptoglobin), accuracy: 109%
(AGP), accuracy: 113%
(Platelet Factor-4), accuracy: 117%
(Adipsin), standard curve range: 0.004-15 ng/mL
(L-Selectin & SAP), standard curve range: 0.012-50 ng/mL
(CRP), standard curve range: 0.037-150 ng/mL
(PF4), standard curve range: 0.061-250 ng/mL
(HPTGN), standard curve range: 0.098-400 ng/mL
(AGP & Adipsin), standard curve range: 0.244-1,000 ng/mL
(Fetuin A & vWF), standard curve range: 0.488-2,000 ng/mL
(A2M)

assay range

accuracy: 88%
(Myoglobin), accuracy: 90%
(P-selectin), accuracy: 98%
(GDF-15), standard curve range: 0.0005-2.5 ng/mL
(GDF-15), standard curve range: 0.012-50 ng/mL
(Lipocalcin-2/NGAL), standard curve range: 0.024-100 ng/mL
(Myoglobin & MPO), standard curve range: 0.085-350 ng/mL
(sICAM-1), standard curve range: 0.122-500 ng/mL
(sVCAM-1), standard curve range: 0.244-1,000 ng/mL
(D-dimer, SAA & P-selectin), standard curve range: 1.221-5,000 ng/mL
(ADAMTS 13), inter-assay cv: <20%
intra-assay cv: <15%

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

General description

The Citrate Synthase Assay Kit contains all the required reagents (including a positive control enzyme) for a fast and simple measurement of citrate synthase activity in a whole cell extract or in isolated mitochondria. In addition, the kit enables testing of the intactness of the mitochondrial inner membrane.

Biochem/physiol Actions

Citrate synthase is the initial enzyme of the tricarboxylic acid (TCA) cycle. The enzyme catalyzes the reaction of 2 carbon acetyl CoA with 4 carbon oxaloacetate to form the 6 carbon citrate. This enzyme is an exclusive marker of the mitochondrial matrix.

Analysis Note

The activity of the enzyme is measured by following the color of TNB, which is generated from DTNB present in the reaction of citrate synthesis, and caused by the deacetylation of Acetyl-CoA. The overall reaction product, TNB, absorbs at 412 nm.

Kit Components Only

Product No.
Description
  • Assay buffer for citrate synthase 5x 25 mL
  • Bicine buffer 10 mL
  • CelLytic™ M Cell Lysis Reagent 10 mL
  • DTNB [5,5'-Dithiobis-(2-nitrobenzoic acid)] 4 mg
  • Acetyl coenzyme A 25 mg
  • Oxaloacetic acid 1 g
  • Citrate synthase (positive control) .1 mL

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H315,H319,H334,H335

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids

Regulatory Information

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    0000189694

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