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Merck
CN

C3228

CelLytic MT Cell Lysis Reagent

For mammalian tissues

Synonym(s):

Cell lysis buffer, Cell lysis reagent

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About This Item

NACRES:
NA.56
UNSPSC Code:
41116134
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Product Name

CelLytic MT Cell Lysis Reagent, For mammalian tissues

form

solution

Quality Level

Analysis Note

It has been tested, but not limited to the following tissues: rat brain, kidney, muscle, heart, liver, spleen and mouse brain, kidney and muscle.

Application

CelLytic MT Cell Lysis Reagent has been used in the homogenization of hippocampi tissues, quadriceps muscles from mice, primary human trophoblasts (PHT), soleus muscles from mice and organs of Corti (OCs).

Features and Benefits

  • Gentle: Non-denaturing and does not interfere with downstream applications
  • Convenient: Provided ready to use

Other Notes

CelLytic MT is an efficient reagent for the extraction of proteins. The lysis buffer consists of a dialyzable mild detergent, bicine, and 150 mM NaCl, resulting in minimal interference with protein interactions and biological activity. CelLytic MT is also used for extraction of cell-line proteins. A volume of 20 mL of CelLytic MT is sufficient for 1 gram of tissue.

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Mechanistic Target of Rapamycin Complex 1 Promotes the Expression of Genes Encoding Electron Transport Chain Proteins and Stimulates Oxidative Phosphorylation in Primary Human Trophoblast Cells by Regulating Mitochondrial Biogenesis
Rosario FJ, et al.
Scientific Reports, 9(1), 246-246 (2019)
Joseph S Marino et al.
The Journal of biological chemistry, 291(50), 25776-25788 (2016-10-28)
Glucocorticoids (GCs) regulate energy supply in response to stress by increasing hepatic gluconeogenesis during fasting. Long-term GC treatment induces hepatic steatosis and weight gain. GC signaling is coordinated via the GC receptor (GR) GRα, as the GRβ isoform lacks a
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de Lima EA, et al.
Journal of Cellular Physiology, 233(12), 96529662-96529662 (2018)
Pasireotide protects mammalian cochlear hair cells from gentamicin ototoxicity by activating the PI3KAkt pathway
Kucharava K, et al.
Cell death & disease, 10(2), 110-110 (2019)

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