Monoclonal Anti-phospho-β-Catenin (pThr⁴¹) antibody produced in mouse

Monoclonal Anti-phospho-β-Catenin (pThr41) (mouse IgG1 isotype) is derived from the BCT-41 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice. β-catenin belongs to the armadillo family and comprises the N-terminal region with 12 imperfect repeats and a conserved C-terminal region. The locus 3p22.1 in the human chromosome harbors β-catenin gene.

Monoclonal Anti-phospho-β-Catenin (pThr41) reacts specifically with human β-catenin phosphorylated at Thr41 (~ 94 kDa).

synthetic phosphopeptide corresponding to amino acids 38-50 (pThr⁴¹) of human β-catenin, conjugated to KLH.

Monoclonal Anti-phospho-β-Catenin (pThr41) antibody produced in mouse may be used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunocytochemistry
• immunoblotting
• immunoprecipitation

β-catenin is a prime component of the wingless (Wnt) pathway and plays a key role in gene expression during development. β-Catenin activity is regulated by phosphorylation on serine/threonine residues located at the protein amino terminus.. The phosphorylation of β-catenin occurs in a protein complex that includes the APC complex (Adenomatous Polyposis Coli), together with Axin by casein kinase I (CKI) and glycogen synthase kinase-3 (GSK-3). The phosphorylation of the β-catenin protein targets the protein to degradation by the β-TrCP (β-transducin repeat-containing protein). Mutations in the serine and threonine residues in the amino-terminal region of the protein and in particular in the phosphorylation sites in that region, cause a reduction in the degradation of the protein and can lead to cancer. Monoclonal antibodies to phospho- β-catenin (pThr41) are an important tool for studying the regulation of β-catenin by phosphorylation and thus the transcription control of many proteins

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

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