Skip to Content
Merck
CN
All Photos(2)

Documents

Safety Information

C7729

Sigma-Aldrich

Anti-Caspase 9 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Sign Into View Organizational & Contract Pricing
All Photos(2)
Synonym(s):
Anti-APAF-3, Anti-APAF3, Anti-ICE-LAP6, Anti-MCH6, Anti-PPP1R56
MDL number:
MFCD01864176
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 35-47 kDa (two bands)

species reactivity

human, rat

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using microwave-treated tissue sections of human and rat heart
immunoprecipitation (IP): 10 μg using HeLa mitochondrial RIPA lysate (500 μg)
microarray: suitable
western blot: 1:300 using whole extract of human Jurkat cells

UniProt accession no.

P55211

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CASP9(842)
rat ... Casp9(58918)

Related Categories

General description

Anti-Caspase 9 is developed in rabbit using a synthetic peptide corresponding to amino acid of human procaspase 9 with N-terminal added lysine conjugated to keyhole limpet hemocyanin (KLH) with glutaraldehyde. Caspase 9/CASP9 (Mch6, ICE-LAP6, APAF3) is a member of the caspase 2 subfamily and is classified together with caspases 8 and 10 as an initiator caspase. It is a cysteine containing aspartate specific protease, made up of 416 amino acids, of which 13 are cysteine. CASP9 is located on human chromosome 1p36.21.

Immunogen

synthetic peptide corresponding to amino acid residues 299-316 of human procaspase 9 with N-terminal added lysine conjugated to KLH with glutaraldehyde.

Application

Anti-Caspase 9 antibody produced in rabbit has been used in western blot analysis.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

Caspase 9/CASP9 is essential for apoptosis during normal development of the murine central nervous system and plays a role in controlling tumor development. It functions as an initiator caspase when mitochondrial dysfunction is the primary event in apoptosis. Caspase 9 functions as an initiator caspase when mitochondrial dysfunction is the primary event in apoptosis. Caspase 9 can also be activated by granzyme B.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Dominika Karolczak et al.
Polish journal of pathology : official journal of the Polish Society of Pathologists, 64(3), 196-203 (2013-10-30)
Aging is the process of progressive accumulation of changes over time, which is additionally connected with increasing susceptibility to some diseases and ultimately leads to death. Aging is associated mainly with loss of permanent cells, e.g. in heart, skeletal muscle
Acidic extracellular pH shifts colorectal cancer cell death from apoptosis to necrosis upon exposure to propionate and acetate, major end-products of the human probiotic propionibacteria
Lan A, et al.
Apoptosis, 12(3), 573-591 (2007)
Genetic screening and functional analysis of CASP 9 mutations in a Chinese cohort with neural tube defects
Liu XZ, et al.
CNS Neuroscience & Therapeutics, 24(5), 394-403 (2018)
Caspase-9
Kuida K
The International Journal of Biochemistry & Cell Biology, 32(2), 121-124 (2000)
Yi Chen et al.
International immunopharmacology, 59, 252-260 (2018-04-19)
This study aimed to explore the role of long non-coding RNA NEAT1 in sepsis-induced acute kidney injury (AKI). The expression levels of NEAT1 in sepsis-induced AKI patients were detected. The rat mesangial cells (RMCs) were treated with lipopolysaccharide (LPS) to
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service