C7207
Monoclonal Anti-β-Catenin antibody produced in mouse
clone 15B8, ascites fluid
Synonym(s):
Monoclonal Anti-β-Catenin
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About This Item
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
15B8, monoclonal
mol wt
antigen 94 kDa
species reactivity
chicken, bovine, human, canine
technique(s)
immunocytochemistry: suitable
immunohistochemistry (frozen sections): suitable
indirect immunofluorescence: 1:1000 using cultured MDBK cells
microarray: suitable
western blot: 1:1000 using cultured MDBK cells extract
isotype
IgG1
UniProt accession no.
application(s)
research pathology
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CTNNB1(1499)
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General description
Monoclonal Anti-β-Catenin (mouse IgG1 isotype) is derived from the 15B8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. β-catenin belongs to the armadillo family and comprises the N-terminal region with 12 imperfect repeats and a conserved C-terminal region. The locus 3p22.1 in the human chromosome harbors β-catenin gene. β-catenin shares 70% sequence identity to a protein encoded by Drosophila armadillo, a segment polarity gene. Both armadillo and β-catenin share considerable homology with plakoglobin, which has been proposed to be γ-catenin.
Specificity
Recognizes specifically mammalian and avian β-catenin. Does not cross-react with plakoglobin (γ-catenin).
Immunogen
recombinant chicken β-catenin.
Application
Monoclonal Anti-β-Catenin antibody produced in mouse has been used:
- immunoblotting
- immunohistochemistry
- immunolabeling
- immunofluorescence
- immunocytochemical staining
Biochem/physiol Actions
β-catenin is a prime component of the wingless (Wnt) pathway and plays a key role in gene expression during development. The homology between β-catenin and armadillo raised the possibility that β-catenin has a developmental signaling role in vertebrates. β-catenin mediates the interaction of the E-cadherin-catenin complex with the epidermal growth factor (EGF) receptor. β-catenin and plakoglobin are substrates for tyrosine phosphorylation following EGF stimulation of cells. β-catenin also associates with the tumor suppressor protein adenomatous polyposis coli (APC). Monoclonal antibody reacting specifically with β-catenin is an essential tool in defining the interactions and distributions of β-catenin and its relationships with other catenins and cadherins in various cells and tissues.
Physical form
The product is provided as ascites fluid with 0.1% sodium azide as a preservative.
Storage and Stability
For extended storage freeze in working aliquots. For continuous use, store at 2-8 °C for up to one month. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Pricing
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Certificates of Analysis (COA)
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