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Safety Information

C6366

SAFC

EX-CELL® CHO Cloning Medium

Animal-component free, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Pharma Manufacturing

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About This Item

UNSPSC Code:
12352207

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Quality Level

sterility

sterile-filtered

product line

EX-CELL®

form

liquid

technique(s)

cell culture | mammalian: suitable (suspension)

components

L-glutamine: no
NaHCO3: yes

shipped in

ambient

storage temp.

2-8°C

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This Item
ACS5050ACS501002ACS503002
pore size

10 kDa NMWCO

pore size

50 kDa NMWCO

pore size

10 kDa NMWCO

pore size

30 kDa NMWCO

manufacturer/tradename

Amicon® Pro

manufacturer/tradename

Amicon® Pro

manufacturer/tradename

Amicon® Pro

manufacturer/tradename

Amicon® Pro

material

Amicon Ultra styrene-butadiene device, polyethylene cap, polypropylene device (for exchange), polypropylene tube (AU0.5; for collection), polypropylene tube (holder), Ultracel® regenerated cellulose membrane (Amicon Ultra-0.5; low binding), polypropylene frit, polyethylene frit, polypropylene tube (for collection)

material

Amicon Ultra styrene-butadiene device, Ultracel® regenerated cellulose membrane (Amicon Ultra-0.5; low binding), polyethylene cap, polyethylene frit, polypropylene device (for exchange), polypropylene frit, polypropylene tube (AU0.5; for collection), polypropylene tube (for collection), polypropylene tube (holder)

material

Amicon Ultra styrene-butadiene device, Ultracel® regenerated cellulose membrane (Amicon Ultra-0.5; low binding), polyethylene cap, polyethylene frit, polypropylene device (for exchange), polypropylene frit, polypropylene tube (AU0.5; for collection), polypropylene tube (for collection), polypropylene tube (holder)

material

Amicon Ultra styrene-butadiene device, Ultracel® regenerated cellulose membrane (Amicon Ultra-0.5; low binding), polyethylene cap, polyethylene frit, polypropylene device (for exchange), polypropylene frit, polypropylene tube (AU0.5; for collection), polypropylene tube (for collection), polypropylene tube (holder)

product line

Amicon®

product line

Amicon®

product line

Amicon®

product line

Amicon®

parameter

10 mL sample volume

parameter

10 mL sample volume

parameter

10 mL sample volume

parameter

10 mL sample volume

General description

Developed to meet the needs of the biotechnology industry, this animal component-free medium is designed for single-cell cloning of recombinant CHO cell lines adapted to serum-free suspension culture.

Application

This animal component-free medium is designed to support clonal survival and growth of Chinese Hamster Ovary (CHO) cell lines, with results comparable to the traditional method using 10% fetal bovine serum. Aseptically add 10 mL of 200 mM L-glutamine solution per 500 mL of medium prior to use.

Legal Information

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

replaced by

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

动植物来源培养基

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Articles

In the present study, we have identifi ed species-specifi c housekeeping genes (HKGs) for Chinese Hamster Ovary (CHO) cells using data from microarray gene expression profiling.

MGAT1 adds N-acetylglucosamine to the Man5GlcNAc2 (Man5) structure. Goh et al. reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells.

A signal peptide is a 5-30 amino acid (aa) peptide present at the N-terminus of secretory proteins.

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