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C3175

Sigma-Aldrich

Chloramphenicol-Water Soluble

powder, suitable for cell culture, BioReagent

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Synonym(s):
Chloramphenicol, Chlornitromycin, Chlorocid, Chloromycetin, Cloramfenicol, D-(−)-threo-2-Dichloroacetamido-1-(4-nitrophenyl)-1,3-propanediol, D-(−)-threo-2,2-Dichloro-N-[β-hydroxy-α-(hydroxymethyl)-β-(4-nitrophenyl)ethyl]acetamide, Chloromycetin, D-threo-2,2-Dichloro-N-[β-hydroxy-α-(hydroxymethyl)-4-nitrophenethyl]aceta, Kloramfenikol
UNSPSC Code:
12352207
NACRES:
NA.76

product line

BioReagent

Quality Level

200

form

powder

technique(s)

cell culture | mammalian: suitable

solubility

H2O: 50-500 mg/mL (stock solution)
PBS: 50-500 mg/mL (stock solution)
other salt solutions: not recommended

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria
mycobacteria
mycoplasma

Mode of action

protein synthesis | interferes

Related Categories

Application

Chloramphenicol-Water Soluble has been used:

  • as a supplement in Luria-Bertani (LB) medium to culture Escherichia coli
  • as a supplement in LB agar to culture Citrobacter rodentium
  • to culture Toxoplasma gondii

Biochem/physiol Actions

Chloramphenicol is a natural product found in Streptomyces venezuelae. It shows a broad range of antibiotic activity against Gram-negative and Gram-positive bacteria by binding to the peptidyl transferase loop of the ribosomal RNA to block the peptide bond formation.

Packaging

Package size based on chloramphenicol

Other Notes

Formulated to contain approx. 100 mg chloramphenicol per gram of powder; balance 2-hydroxypropyl-β-cyclodextrin.
Keep container tightly closed in a dry and well-ventilated place. Light sensitive. Keep in a dry place.

Pictograms

Health hazardCorrosion
GHS08,GHS05

Signal Word

Danger

Hazard Statements

H318,H351,H361fd

Hazard Classifications

Carc. 2 - Eye Dam. 1 - Repr. 2

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

监管及禁止进口产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5.  How do I calculate the molarity of product C3175, Chloramphenicol-Water Soluble?

    Product C3175 (Chloramphenicol-Water Soluble) is a complex of chloramphenicol and cyclodextrin. Cyclodextrin is used to aid in the solubility of chloramphenicol into water. In order to prepare a molar solution of chloramphenicol you will have to base it on the chloramphenicol content in the solution and not the entire complex.  Product C3175 is formulated to contain approximately 100 mg chloramphenicol per gram of powder.  Based on this average, if you are preparing a 500 mg/ml stock solution, there will be approximately 50 mg of chloramphenicol in 1 ml.  Below is the calculated molarity of chloramphenicol when the complex is dissolved at 500 mg/ml in water. molecular weight X unknown molarity X volume = complex weight X complex ratio323.13 g/mol X Molarity X 1 ml = 500 mg X 0.1           Molarity = 0.155 Mchloramphenicol molecular weight = 323.13 g/molmolarity (unknown) = mol/Lvolume = 1mlcomplex weight = 500 mgcomplex ratio = 100 mg chloramphenicol/1 gm complex

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A Chatzitakis et al.
Water research, 42(1-2), 386-394 (2007-08-19)
The photocatalytic degradation of Chloramphenicol, an antibiotic drug, has been investigated in aqueous heterogeneous solutions containing n-type oxide semiconductors as photocatalysts. The disappearance of the organic molecule follows approximately a pseudo-first-order kinetics according to the Langmuir-Hinshelwood model. It was observed
Jaap C Hanekamp et al.
Environmental toxicology and pharmacology, 39(1), 213-220 (2014-12-22)
The antibiotic chloramphenicol (CAP) is banned from food production. Besides being a medicinal product, CAP is also a natural product, produced by Streptomyces Venezuelae. The lack of scientific data hampers setting of an Acceptable Daily Intake (ADI). Consequently, a maximum
D H Burke et al.
Chemistry & biology, 4(11), 833-843 (1998-01-31)
The problem of how macromolecules adopt specific shapes to recognize small molecules in their environment is readily addressed through in vitro selections (the SELEX protocol). RNA-antibiotic interactions are particularly attractive systems for study because they provide an opportunity to expand
Taru Hilander et al.
Nucleic acids research, 46(2), 849-860 (2017-12-12)
Accuracy of protein synthesis is enabled by the selection of amino acids for tRNA charging by aminoacyl-tRNA synthetases (ARSs), and further enhanced by the proofreading functions of some of these enzymes for eliminating tRNAs mischarged with noncognate amino acids. Mouse
Yehou M D Gnopo et al.
Journal of colloid and interface science, 578, 522-532 (2020-06-17)
Recombinantly engineered bacterial outer membrane vesicles (OMVs) are promising vaccine delivery vehicles. The diversity of exogenous antigens delivered by OMVs can be enhanced by induced fusion of OMV populations. To date there are no reports of induced fusion of bacterial
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