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C2278

Sigma-Aldrich

CAPSO

≥99% anhydrous basis (titration)

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Synonym(s):
3-(Cyclohexylamino)-2-hydroxy-1-propanesulfonic acid, CAPSO Free Acid
Empirical Formula (Hill Notation):
C9H19NO4S
CAS Number:
73463-39-5
Molecular Weight:
237.32
Beilstein:
5939234
MDL number:
MFCD00041778
UNSPSC Code:
12161700
PubChem Substance ID:
24892483
NACRES:
NA.25

Quality Level

200

Assay

≥99% anhydrous basis (titration)

form

crystalline powder

pH

2.5-4.0

useful pH range

8.9-10.3

pKa (25 °C)

9.6

solubility

water: 0.111 g/mL, clear, colorless

application(s)

diagnostic assay manufacturing

SMILES string

OC(CNC1CCCCC1)CS(O)(=O)=O

InChI

1S/C9H19NO4S/c11-9(7-15(12,13)14)6-10-8-4-2-1-3-5-8/h8-11H,1-7H2,(H,12,13,14)

InChI key

INEWUCPYEUEQTN-UHFFFAOYSA-N

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General description

CAPSO is a useful secondary standard buffer. Second dissociation constant pK2 value of CAPSO has been measured. Useful pH range for CAPSO is 8.9 - 10.3.

Application

CAPSO was used as biological buffer in the following studies:
  • To maintain pH 9 during water leach test for the analysis of high-carbon coal fly ash-soil mixtures.
  • Chemiluminescent in situ hybridization using soybean peroxidase-labeled peptide nucleic acid (PNA) probes. I
  • Preparation of planar bilayer lipid membranes.
It may be employed as buffer for the electrophoretic analyses in non-denaturing gels, to investigate macromolecular self-association of ADP-ribosyltransferase protein in solution.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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H Stender et al.
Applied and environmental microbiology, 67(1), 142-147 (2001-01-03)
A new chemiluminescent in situ hybridization (CISH) method provides simultaneous detection, identification, and enumeration of culturable Escherichia coli cells in 100 ml of municipal water within one working day. Following filtration and 5 h of growth on tryptic soy agar
P I Bauer et al.
The Biochemical journal, 270(1), 17-26 (1990-08-15)
The macromolecular self-association of ADP-ribosyltransferase protein in solution was studied by several experimental techniques: quantitative gel filtration, electrophoretic analyses in non-denaturing gels, and cross-linking the enzyme protein with glutaraldehyde, dimethyl pimelimidate, dimethyl suberimidate, dimethyl 3,3'-dithiobisproprionimidate and tetranitromethane. The self-association of
Evaluation of leaching protocols for testing of high-carbon coal fly ash-soil mixtures.
Becker J, et al.
Journal of Environmental Engineering (New York, N.Y.), 139(5), 642-653 (2012)
Second dissociation constants of 3-(cyclohexylamino)-1-propanesulfonic acid (CAPS) and 3-(cyclohexylamino)-2-hydroxy-1-propanesulfonic acid (CAPSO) from the temperatures 278.15 K to 328.15 K.
Roy RN, et al.
The Journal of Chemical Thermodynamics, 29(7), 749-756 (1997)
Andreas Missner et al.
The Journal of biological chemistry, 283(37), 25340-25347 (2008-07-12)
Several membrane channels, like aquaporin-1 (AQP1) and the RhAG protein of the rhesus complex, were hypothesized to be of physiological relevance for CO(2) transport. However, the underlying assumption that the lipid matrix imposes a significant barrier to CO(2) diffusion was
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