C1374
CDK6/CyclinD1, active, His/GST tagged human
PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
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Synonym(s):
CCND1, Cell division protein kinase 6, BCL1, CDK6, D11S287E, MGC59692, PLSTIRE, PRAD1, U21B31
UNSPSC Code:
12352200
NACRES:
NA.32
Recommended Products
recombinant
expressed in baculovirus infected Sf9 cells
Quality Level
product line
PRECISIO® Kinase
Assay
≥70% (SDS-PAGE)
form
buffered aqueous glycerol solution
specific activity
7.9-10.7 nmol/min·mg
mol wt
CDK6 subunit ~40 kDa
cyclin D1 ~61 kDa
technique(s)
activity assay: suitable
solubility
soluble
water: soluble
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... CCND1(595) , CDK6(1021)
General description
Research area: APOPTOSIS
This recombinant product was expressed by baculovirus in Sf9 insect cells using an N-terminal His-tag on CDK6, and a GST-tag on Cyclin D1.
This recombinant product was expressed by baculovirus in Sf9 insect cells using an N-terminal His-tag on CDK6, and a GST-tag on Cyclin D1.
Application
CDK6/CyclinD1, active, His/GST tagged human has been used in: In vitro CDK6 activity assay to evaluate the effects of small-molecule inhibitors for therapeutic haematopoietic stem cells (HSC) expansion.
Biochem/physiol Actions
Cyclin Dependent Kinases6 (CDK6) is a member of the cyclin-dependent family of protein kinases that are important regulators of cell cycle progression. CDK6 activity is regulated by the D-type cyclins and members of the INK4 family of CDK inhibitors. CDK6 kinase activity is detected during the mid-G1 phase of the cell cycle. It plays a crucial role in phosphorylating and regulating the activity of the retinoblastoma (RB), a tumor suppressor protein. Moreover, CDK6 has active involvement during transcription in hematopoietic stem cells (HSC) and tumor cells. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivity in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein. The activation of cyclin D1-dependent kinase activity is found to be necessary to trigger the process of apoptosis.
Physical form
Supplied in 50 mM Sodium Phosphate, pH 7.0, with 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, and 25% glycerol.
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Certificates of Analysis (COA)
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Ectopic expression of cyclin D1 impairs the proliferation and enhances the apoptosis of a murine lymphoid cell line
Duquesne F, et al.
Cell Death and Differentiation, 8(1), 51-62 (2001)
CDK6-a review of the past and a glimpse into the future: from cell-cycle control to transcriptional regulation
Tigan A-S, et al.
Oncogene, 35(24), 3083-3091 (2016)
Tohru Takaki et al.
Journal of biochemistry, 137(3), 381-386 (2005-04-06)
D-type cyclin-dependent kinases (Cdk4 and Cdk6) regulate the G1 to S phase progression of the mammalian cell cycle. It has been suggested that Cdk4 and Cdk6 may have distinct functions in vivo, even though they are indistinguishable biochemically. Here we
M Meyerson et al.
Molecular and cellular biology, 14(3), 2077-2086 (1994-03-01)
A family of vertebrate cdc2-related kinases has been identified, and these kinases are candidates for roles in cell cycle regulation. Here, we show that the human PLSTIRE gene product is a novel cyclin-dependent kinase, cdk6. The cdk6 kinase is associated
Haizhen Wang et al.
Nature, 546(7658), 426-430 (2017-06-14)
D-type cyclins (D1, D2 and D3) and their associated cyclin-dependent kinases (CDK4 and CDK6) are components of the core cell cycle machinery that drives cell proliferation. Inhibitors of CDK4 and CDK6 are currently being tested in clinical trials for patients
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