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B3133

Sigma-Aldrich

Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride

≥99% (TLC), suitable for substrate for trypsin

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Synonym(s):
L-BAPA, L-BAPNA, BANI
Empirical Formula (Hill Notation):
C19H22N6O4 · HCl
CAS Number:
Molecular Weight:
434.88
Beilstein:
4081878
EC Number:
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.32

Quality Level

Assay

≥99% (TLC)

form

powder

solubility

acetone: water (1:1): 50 mg/mL

suitability

suitable for substrate for trypsin

storage temp.

−20°C

SMILES string

Cl[H].NC(=N)NCCC[C@H](NC(=O)c1ccccc1)C(=O)Nc2ccc(cc2)[N+]([O-])=O

InChI

1S/C19H22N6O4.ClH/c20-19(21)22-12-4-7-16(24-17(26)13-5-2-1-3-6-13)18(27)23-14-8-10-15(11-9-14)25(28)29;/h1-3,5-6,8-11,16H,4,7,12H2,(H,23,27)(H,24,26)(H4,20,21,22);1H/t16-;/m0./s1

InChI key

DEOKFPFLXFNAON-NTISSMGPSA-N

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General description

Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride is a substrate for trypsin.

Application

Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride has been used as a substrate:
  • for chymotrypsin and trypsin activity assay in enzymatic extract (EE) of M. tenellum and pancreatic tissue homogenates
  • in trypsin and papain standard curve generation
  • in serine protease and fibrinogenolytic assays of eupolytin1 protein

Linkage

Similar to B3279, but produced for Sigma.

Substrates

Chromogenic substrate for trypsin and other proteolytic enzymes.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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