A9469
Monoclonal ANTI-FLAG® M2-Alkaline Phosphatase antibody produced in mouse
clone M2, purified immunoglobulin, buffered aqueous glycerol solution
Synonym(s):
Monoclonal ANTI-FLAG® M2, Anti-ddddk, Anti-dykddddk
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About This Item
NACRES:
NA.32
UNSPSC Code:
41106514
Conjugate:
alkaline phosphatase conjugate
Clone:
M2, monoclonal
Application:
ELISA (i)
Citations:
54
biological source
mouse
conjugate
alkaline phosphatase conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
M2, monoclonal
form
buffered aqueous glycerol solution
species reactivity
all
concentration
~1 mg/mL
technique(s)
indirect ELISA: 1:20,000
isotype
IgG1
immunogen sequence
DYKDDDDK
shipped in
wet ice
storage temp.
−20°C
Quality Level
Gene Information
human ... ALPL(249)
Related Categories
General description
Monoclonal ANTI-FLAG® M2-Alkaline Phosphatase is a purified IgG 1 mouse antibody covalently conjugated to calf intestinal alkaline phosphatase (AP). The antibody conjugate binds to FLAG® fusion proteins and will recognize the FLAG® epitope at any position in the fusion protein (N-terminal, Met-N-terminal, C-terminal or internal FLAG® peptides).
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Monoclonal ANTI-FLAG® M2-Alkaline Phosphatase antibody produced in mouse has been used:
- in direct tissue blot immunoassay of sweet orange petioles samples
- in screening internalization of delta opioid receptor
- for screening cell-free protein expression using ELISA
Physical form
Solution in Tris buffered saline containing 50% glycerol plus stabilizer and preservative
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
常规特殊物品
低风险生物材料
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Beth A Rasala et al.
Plant biotechnology journal, 9(6), 674-683 (2011-05-04)
Microalgae have the potential to be a valuable biotechnological platform for the production of recombinant proteins. However, because of the complex regulatory network that tightly controls chloroplast gene expression, heterologous protein accumulation in a wild-type, photosynthetic-competent algal chloroplast remains low.
Integration of cell-free protein coexpression with an enzyme-linked immunosorbent assay enables rapid analysis of protein-protein interactions directly from DNA
Layton CJ and Helling HW
Protein Science, 20(8), 1432-1438 (2011)
The delta opioid receptor positive allosteric modulator BMS 986187 is a G protein biased allosteric agonist
Stanczyk MA, et al.
British Journal of Pharmacology (2019)
Mun Kyoung Kim et al.
Molecular and cellular biology, 30(10), 2411-2423 (2010-03-10)
The small nuclear RNA (snRNA)-activating protein complex (SNAPc) is essential for transcription of genes coding for the snRNAs (U1, U2, etc.). In Drosophila melanogaster, the heterotrimeric DmSNAPc recognizes a 21-bp DNA sequence, the proximal sequence element A (PSEA), located approximately
Jiandong Chen et al.
Genes & development, 31(13), 1382-1395 (2017-08-11)
Mismatch repair (MMR) is a conserved mechanism exploited by cells to correct DNA replication errors both in growing cells and under nongrowing conditions. Hfq (host factor for RNA bacteriophage Qβ replication), a bacterial Lsm family RNA-binding protein, chaperones RNA-RNA interactions
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