A8111
Plasminogen activator inhibitor 1 (PAI-1)
≥200,000 units/mg protein, lyophilized powder, ≥90% (SDS-PAGE)
Synonym(s):
PAIE, PLANH1, Plasminogen activator inhibitor, β-migrating endothelial cell-derived type, Serpin E1 PAI-1, Serpine 1
Product Name
Plasminogen activator inhibitor 1 (PAI-1) human, recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
recombinant
expressed in E. coli
assay
≥90% (SDS-PAGE)
form
lyophilized powder
specific activity
≥200,000 units/mg protein
mol wt
~43 kDa
solubility
water: 0.1 mL, clear, colorless
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Quality Level
Gene Information
human ... SERPINE1(5054)
General description
Plasminogen activator inhibitor 1 (PAI-1) or serine protease inhibitor E1 (SERPINE1) gene is mapped to human chromosome 7q22.1. PAI-1 is produced in hepatocytes, endothelial cells, smooth muscle cells, megakaryocytes, and adipocytes.
Application
Plasminogen activator inhibitor 1 (PAI-1) human has been used:
- as an inhibitor of pro-brain-derived neurotrophic factor (proBDNF) in radioimmunoprecipitation assay (RIPA) in peripheral blood and lymphocytes
- to block plasmin activation in mice infected with C. albicans hyphae
- to test its effect on neurite growth in neurons
Biochem/physiol Actions
Plasminogen Activator Inhibitor 1 (PAI-1) protein belongs to the Serpin superfamily of serine protease inhibitors. It is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), the activators of plasminogen and therefore inhibits the fibrinolysis process. PAI is involved in extracellular matrix remodeling and has been implicated in processes like angiogenesis, chemotaxis, ovulation, and embryogenesis. PAI-1 is present in increased levels in various disease states such as a number of cancer forms, obesity and the metabolic syndrome.
Plasminogen activator inhibitor 1 (PAI-1), a member of the Serpin superfamily of serine protease inhibitors, is involved in extracellular matrix remodeling and implicated in processes like angiogenesis, chemotaxis, ovulation, and embryogenesis.
Plasminogen activator inhibitor–1 (PAI -1) participates in mediating cellular senescence. It is upregulated during injury along with other tissue repair transcriptomes. Elevated levels of PAI-1 is regarded as one of the risks associated with myocardial infarction (MI).
Physical form
Supplied as a lyophilized powder from a solution containing 0.5 M sodium chloride, 20 mM sodium acetate, and 50 mg/mL trehalose.
Preparation Note
Reconstitute to 0.25 mg/ml with ultrapure water. The reconstituted product contains 0.25 mgP/mL in 0.5 M sodium chloride, 20 mM sodium acetate, and 50 mg/mL trehalose.
Analysis Note
The product is the active human PAI-1 protein with a molecular weight of 43 kDa.
Other Notes
1 unit irreversibly inhibits (100% inhibition) 1 unit of tissue plasminogen activator (tPA) under physiological conditions (neutral pH and 37 ºC).
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Laura Yunes-Medina et al.
Molecular and cellular neurosciences, 86, 72-80 (2017-12-05)
The protein transglutaminase 2 (TG2) has been implicated as a modulator of neuronal viability. TG2's role in mediating cell survival processes has been suggested to involve its ability to alter transcriptional events. The goal of this study was to examine
Matteo Cesari et al.
Cardiovascular therapeutics, 28(5), e72-e91 (2010-07-16)
The close relationship existing between aging and thrombosis has growingly been studied in this last decade. The age-related development of a prothrombotic imbalance in the fibrinolysis homeostasis has been hypothesized as the basis of this increased cardiovascular and cerebrovascular risk.
William Santus et al.
Science immunology, 2(15) (2017-09-25)
Nuclear factor of activated T cells (NFAT) is activated in innate immune cells downstream of pattern recognition receptors, but little is known about NFAT's functions in innate immunity compared with adaptive immunity. We show that early activation of NFAT balances
Sadiya S Khan et al.
Science advances, 3(11), eaao1617-eaao1617 (2017-11-21)
Plasminogen activator inhibitor-1 (PAI-1) has been shown to be a key component of the senescence-related secretome and a direct mediator of cellular senescence. In murine models of accelerated aging, genetic deficiency and targeted inhibition of PAI-1 protect against aging-like pathology
Liying Lin et al.
Journal of psychiatric research, 133, 166-173 (2020-12-21)
Previous studies showed that blood BDNF levels in mood disorders were reduced. However, little is known about the changes of BDNF and its precursor proBDNF in lymphocytes. In addition, earlier studies using commercial ELISA kits cannot distinguish mature BDNF from
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