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Safety Information

A7653

Sigma-Aldrich

L-Alanine Dehydrogenase from Bacillus subtilis

buffered aqueous glycerol solution, ~30 units/mg protein (Lowry)

Synonym(s):

L-Alanine: NAD+ oxidoreductase (deaminating)

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250 ML
CN¥2,105.25

CN¥2,105.25

Estimated to ship onNovember 17, 2025Details

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250 ML
CN¥2,105.25

About This Item

CAS Number:
9029-06-5
Enzyme Commission number:
1.4.1.1 (BRENDA, IUBMB)
EC Number:
232-847-9
MDL number:
MFCD00131441
UNSPSC Code:
12352204
NACRES:
NA.54

CN¥2,105.25

Estimated to ship onNovember 17, 2025Details

biological source

Bacillus subtilis

Quality Level

200

form

buffered aqueous glycerol solution

specific activity

~30 units/mg protein (Lowry)

foreign activity

LDH ~1% (using pyruvate as substrate)

storage temp.

−20°C

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Show Differences

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This Item
I85071935105S06052
碘甲烷 (stabilised) for synthesis

806064

碘甲烷

碘甲烷 contains copper as stabilizer, ReagentPlus®, 99%

I8507

碘甲烷

碘甲烷 SAJ first grade, ≥93.0%

193510

碘甲烷

碘甲烷 2000 μg/mL in methanol: water (4:1), analytical standard

5S06052

碘甲烷

vapor pressure

24.09 psi ( 55 °C)

vapor pressure

24.09 psi ( 55 °C), 7.89 psi ( 20 °C)

vapor pressure

24.09 psi ( 55 °C), 7.89 psi ( 20 °C)

vapor pressure

-

assay

≥99.0% (GC)

assay

99%

assay

≥93.0%

assay

-

form

liquid

form

liquid

form

liquid

form

-

potency

79.84 mg/kg LD50, oral (rat)

potency

-

potency

-

potency

-

expl. lim.

8.5-66 % (v/v)

expl. lim.

-

expl. lim.

-

expl. lim.

-

Application

L-Alanine dehydrogenase converts L-alanine to pyruvate and ammonium. L-Alanine dehydrogenase from Bacillus subtilis may be used to study enzyme inactivation and protection [1].

Biochem/physiol Actions

L-Alanine dehydrogenase is an A-stereospecific dehydrogenase that catalyzes the reversible deamination of L-alanine to pyruvate and ammonium. It is important for the generation of pyruvate during sporulation. L-Alanine dehydrogenase from Bacillus subtilis has a predominately ordered kinetic mechanism in which NAD binds before L-alanine. Subsequently, ammonia, pyruvate, and NADH are released in that specific order.[1] Optimal pH for the amination reaction is 8.8-9.0, whereas it is 10-10.5 for the deamination reaction. The enzyme is inactivated by divalent metal ions and p-chloromercuribenzoate, mercuric ion being most effective. The inactivation may be reversed by L- or D-cysteine.[2]

Unit Definition

One unit will convert 1.0 μmole of L-alanine to pyruvate and NH3 per min at pH 10.0 at 25 °C.

Physical form

Solution in 50% glycerol containing 10 mM potassium phosphate buffer, pH 7.7

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000380060
    0000279721
    0000380060
    0000279721
    11181151

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    Hexigeduleng Bao et al.
    Plant, cell & environment, 38(3), 600-613 (2014-07-31)
    γ-Aminobutyric acid (GABA) accumulates in many plant species in response to environmental stress. However, the physiological function of GABA or its metabolic pathway (GABA shunt) in plants remains largely unclear. Here, the genes, including glutamate decarboxylases (SlGADs), GABA transaminases (SlGABA-Ts) and
    Wei Ye et al.
    Microbiological research, 165(4), 268-275 (2009-09-02)
    The major objective of the present study is to change the alanine production of Lactic acid bacteria by expression of Bacillus subtilis (natto) alanine dehydrogenase (AlaDH), the gene that is not present in Lactic acid. B. subtilis AlaDH gene (ald)
    Gabriella M Scandurra et al.
    Microbiology and immunology, 50(10), 805-810 (2006-10-21)
    The tuberculosis (TB) vaccine strain Mycobacterium bovis BCG is unable to utilise alanine and this deficiency is thought to inhibit the growth of the vaccine in vivo and limit vaccine efficacy. In this report we demonstrate that L-alanine catabolism can
    Crystal structures of the Mycobacterium tuberculosis secretory antigen alanine dehydrogenase (Rv2780) in apo and ternary complex forms captures "open" and "closed" enzyme conformations.
    Sarvind Mani Tripathi et al.
    Proteins, 72(3), 1089-1095 (2008-05-21)
    Karin Denger et al.
    Microbiology (Reading, England), 152(Pt 11), 3197-3206 (2006-11-01)
    A degradative pathway for taurine (2-aminoethanesulfonate) in Rhodobacter sphaeroides 2.4.1 was proposed by Brüggemann et al. (2004) (Microbiology 150, 805-816) on the basis of a partial genome sequence. In the present study, R. sphaeroides 2.4.1 was found to grow exponentially
    View All Related Papers

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