A6103
Aprotinin
recombinant, expressed in Nicotiana (tobacco), ≥5 TIU/mg protein, powder, ≥98% (SDS-PAGE), suitable for inhibition assay
Synonym(s):
BPTI, Basic pancreatic trypsin inhibitor, Kallikrein-trypsin inactivator, Kunitz protease inhibitor
About This Item
Recommended Products
Product Name
Aprotinin Nicotiana tobacco, >= 5TIU/mg protein, >= 98 % SDS-PAGE | 9087-70-1, recombinant, expressed in Nicotiana (tobacco), ≥5 TIU/mg protein, ≥98% (SDS-PAGE)
recombinant
expressed in Nicotiana (tobacco)
Quality Level
description
recombinant form of thenative bovine-sequence aprotinin purified from plant tissue (tobacco)
product line
BioUltra
Assay
≥98% (SDS-PAGE)
form
powder
specific activity
≥5 TIU/mg protein
mol wt
~_6.5 kDa
technique(s)
inhibition assay: suitable
solubility
0.85% sodium chloride: 5 mg/mL
UniProt accession no.
storage temp.
2-8°C
Gene Information
cow ... PTI(404172)
Unit Definition
Preparation Note
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Regulatory Information
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How does the storage temperature relate to shipping conditions?
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
How does Product A6103, Aprotinin bovine, compare to Trasylol?
Trasylol is the Bayer AG registered trademark name for this material.
Do you assay this the activity of Product A6103, Aprotinin bovine, in TIU or in KIU?
We express the product's activity in TIU (Trypsin Inhibitor Units). Multiply the activity on the Certificate of Analysis by 1300 to convert it to KIU (Kallikrein Inhibitor Units).
How should I store solutions of Product A6103, Aprotinin bovine?
We generally advise storing stock solutions of approximately 5-10 mg/mL. Working solutions down to 0.065 micrograms/mL can be stored refrigerated at 2-8°C for one week, or at - 20°C for appoximately six months in single use aliquots. See Table 2 in the Datasheet for details.
At what concentration should I use Product A6103, Aprotinin bovine?
For most applications, 10 to 800 nM works well (based on a molecular weight of 6512 g/mol). This information may be found in the technical bulletin for the Protease Inhibitor Panel, Product No. INHIB1.
Product A6103, recombinant bovine aprotinin has a specification of ~60% protein. What is the remaining components of the solid?
Product A6103, recombinant bovine aprotinin is >98% purity by SDS-PAGE. The product is ~60% protein with the balance being salts (NaCl).
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Articles
While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.
ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.
Protocols
Objective: To standardize a procedure for the enzymatic assay of Aprotinin.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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