A5979
Anti-ARP3 antibody, Mouse monoclonal
clone FMS338, purified from hybridoma cell culture
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
FMS338, monoclonal
form
buffered aqueous solution
mol wt
antigen 40-45 kDa
species reactivity
human, mouse, rat, canine
packaging
antibody small pack of 25 μL
technique(s)
immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.25-0.5 μg/mL using total cell extract of mouse myoblast C2 cells
isotype
IgG2b
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ACTR3(10096)
mouse ... Actr3(74117)
rat ... Actr3(81732)
General description
Monoclonal Anti-ARP3 (mouse IgG2b isotype) is derived from the hybridoma FMS338 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with recombinant human ARP3. Arp3 is mapped to human chromosome 2q14.1.
Immunogen
recombinant human ARP3.
Application
Monoclonal Anti-ARP3 antibody produced in mouse has been used in:
- enzyme linked immunosorbent assay (ELISA)
- immunocytochemistry
- immunoblotting
- immunoprecipitation
- immunofluorescence
- immunohistochemistry
Biochem/physiol Actions
The ARP2/3 complex consists of seven distinct proteins (Arp2 and Arp3, p41-Arc, p34-Arc, p21-Arc, p20-Arc, and p16-Arc) that modulate polymerization of cellular actin filaments. ARP3 regulates actin nucleation, and is involved in directing the movement of Listeria monocytogenes in infected host cells. Monoclonal Anti-ARP3 antibody binds to human, canine, rat, and mouse ARP3.
The actin-related protein-2/3 (ARP2/3) is part of Wiskott-Aldrich Syndrome protein (WASP) signaling in mediating polymerization state of actin filaments. It has been found that ARP2/3 complex and its activator spinocerebellar ataxia, autosomal recessive 2 (Scar2) are involved in Golgi polarization in NIH 3T3 cells. However in primary astrocytes, Golgi polarization involves neither actin cytoskeleton or Arp2/3 complex nor any WASP-family of proteins. In a different model, it was found that the ARP2/3 complex was necessary for neutrophil chemotaxis and phagocytosis. By genetic and loss of function studies, it was demonstrated that Scar2 is the major regulator of the ARP2/3 complex rather than the WASP family of proteins, the latter having more restricted roles in specific cellular events.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Certificates of Analysis (COA)
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Identification of the p16-Arc subunit of the Arp 2/3 complex as a substrate of MAPK-activated protein kinase 2 by proteomic analysis
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