CBL221
Anti-RNA Polymerase II Antibody, clone ARNA-3
ascites fluid, clone ARNA-3, Chemicon®
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Synonym(s):
DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, RNA polymerase II subunit B1
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
ascites fluid
antibody product type
primary antibodies
clone
ARNA-3, monoclonal
species reactivity
kangaroo, human, Drosophila
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... POLR2A(5430)
General description
DNA-directed RNA polymerase II subunit RPB1 (EC 2.7.7.6; UniProt P04052; also known as DNA-directed RNA polymerase III largest subunit, RNA polymerase II subunit B1) is encoded by the RpII215 (also known as CG1554) gene (Gene ID 32100) in drosophila melanogaster (fruit fly). RNA polymerase II (Pol II) is a multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.
Specificity
ARNA-3 is excellent for detection of transcription activity, e.g. puffs in polytene chromosomes. Reacts with RNA polymerase II in biological materials. Reactive polypeptides after SDS-PAGE: 200 kDa band of nonphosphorylated and 240 kDa band of phosphorylated Pol IIA.
Broad species cross-reactivity expected due to sequence homology.
Immunogen
Epitope: Amino acids 794-822
Purified Drosophila RNA polymerase II
Application
Detect RNA Polymerase II with Anti-RNA Polymerase II Antibody, clone ARNA-3 (Mouse Monoclonal Antibody), that has been shown to work in WB & IHC.
Western blot
Immunohistochemistry: 1:100; Frozen tissue; fixation recommendation - incubate tissue with 2% paraformaldehyde in PBS for 10 min, wash in PBS for 5 min, incubate in 0.2% Triton X-100 in PBS for 5 min, wash in PBS for 5 min. Incubate with antibody one hour.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry: 1:100; Frozen tissue; fixation recommendation - incubate tissue with 2% paraformaldehyde in PBS for 10 min, wash in PBS for 5 min, incubate in 0.2% Triton X-100 in PBS for 5 min, wash in PBS for 5 min. Incubate with antibody one hour.
Optimal working dilutions must be determined by the end user.
Physical form
Supplied as hybridoma culture supernatant concentrate containing 0.09% sodium azide.
Analysis Note
Control
Positive for drosophila, kangaroo kidney epithelial cell line PtK2, and human HeLa and HEp-2 cell lines.
Positive for drosophila, kangaroo kidney epithelial cell line PtK2, and human HeLa and HEp-2 cell lines.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
Certificates of Analysis (COA)
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Tim Patrick Kaminski et al.
Molecular biology of the cell, 25(14), 2190-2198 (2014-05-23)
Regulation of RNA polymerase II (RNAPII) during transcription is essential for controlling gene expression. Here we report that the transcriptional activity of RNAPII at the Balbiani ring 2.1 gene could be halted during stable elongation in salivary gland cells of
Shih-Ying Tsai et al.
Epigenetics & chromatin, 9, 32-32 (2016-07-29)
Genome-wide studies in higher eukaryotes have revealed the presence of paused RNA polymerase II (RNA-Pol) at about 30-50 bp downstream of the transcription start site of genes involved in developmental control, cell proliferation and intercellular signaling. Promoter-proximal pausing is believed to
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