A0480
Anti-Actin (plant) antibody, Mouse monoclonal
clone 10-B3 (MAbGPa), purified from hybridoma cell culture
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Synonym(s):
Monoclonal Anti-Actin (plant) antibody produced in mouse
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
10-B3 (MAbGPa), monoclonal
mol wt
antigen ~45 kDa
species reactivity
plant
packaging
antibody small pack of 25 μL
technique(s)
immunohistochemistry: suitable
indirect ELISA: suitable
western blot: 1-2 μg/mL using corn leaves extracts
isotype
IgG2b
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
Arabidopsis thaliana ... ACT8(841347)
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General description
Arabidopsis has a complex actin gene family that encodes six protein subclasses. One of the classes is represented by the proteins ACT2 and ACT8, which are similar proteins, but have different flanking sequences, introns and silent nucleotide positions in codons. ACT8 is expressed in root, stems, leaves, flowers, pollen and siliques.
Monoclonal Anti-Actin (plant) antibody recognizes actin in several plant species including Dictyostelium. In Arabidopsis, the antibody recognizes all eight actins isoforms (ACT1, 2, 3, 4, 7, 8, 11 and 12).
Monoclonal Anti-Actin (plant) antibody recognizes actin in several plant species including Dictyostelium. In Arabidopsis, the antibody recognizes all eight actins isoforms (ACT1, 2, 3, 4, 7, 8, 11 and 12).
Monoclonal Anti-Actin (plant) (mouse IgG2b isotype) is derived from the hybridoma 10-B3 (MabGPa) produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice immunized with ACT8 protein. In Arabidopsis, actin is divided into two major phylogenetic classes namely reproductive and vegetative class.
Immunogen
ACT8 protein.
Application
Monoclonal Anti-Actin (plant) antibody produced in mouse has been used in:
- two-layer phos-tag polyacrylamide gel electrophoresis (PAGE)
- immunohistochemistry and enzyme-linked immunosorbent assay (ELISA).
- immunoblotting
Biochem/physiol Actions
In Arabidopsis ACT7 isovariant is essential for normal phytohormone response to hormones in different organs. Mutation in the actin (ACT)1 gene is associated with the development of dwarfism in plants in addition to delayed flowering, highly reduced organ size and varied branching pattern of the leaf trichomes and inflorescence stem. In plants, there is a developmental switch in the modulation of actin isovariants throughout cell differentiation and maturation phase.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Jodi L Stewart et al.
PloS one, 6(5), e19894-e19894 (2011-06-01)
As photoautotrophs, plants can use both the form and amount of fixed carbon as a measure of the light environment. In this study, we used a variety of approaches to elucidate the role of exogenous sucrose in modifying seedling growth
Hong-Xian Hsing et al.
Botanical studies, 57(1), 30-30 (2017-06-10)
Phalaenopsis orchid (Phal. orchid) is visually attractive and it is important economic floriculture species. Phal. orchids have many unique biological features. However, investigation of these features and validation on their biological functions are limited due to the lack of an
An Endogenous Carbon-Sensing Pathway Triggers Increased Auxin Flux and Hypocotyl Elongation
Lilley JL, et al.
Plant Physiology, 160(4), 2261-2270 (2012)
Y Q An et al.
The Plant journal : for cell and molecular biology, 10(1), 107-121 (1996-07-01)
Arabidopsis has a complex and ancient actin gene family encoding six divergent subclasses of proteins. One subclass is represented by ACT2 and ACT8, which encode nearly identical proteins. These two genes differ significantly in flanking and intron sequences and in
Functional Nonequivalency of Actin Isovariants in
Kandasamy, et al.
Molecular Biology of the Cell, 13(1), 251-251 (2002)
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