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Merck
CN

94875

Atto 680

BioReagent, suitable for fluorescence, ≥90% (HPLC)

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About This Item

MDL number:
MFCD03456138
UNSPSC Code:
12352116
PubChem Substance ID:
93577787
NACRES:
NA.32

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Product Name

Atto 680, BioReagent, suitable for fluorescence, ≥90% (HPLC)

InChI

1S/C27H31N3O6S/c1-4-29-9-5-7-17-11-20-24(13-22(17)29)36-25-14-23-19(12-21(25)28-20)18(16-37(33,34)35)15-27(2,3)30(23)10-6-8-26(31)32/h11-15H,4-10,16H2,1-3H3,(H-,31,32,33,34,35)

InChI key

MHHMNDJIDRZZNT-UHFFFAOYSA-N

product line

BioReagent

assay

≥90% (HPLC)

form

powder

manufacturer/tradename

ATTO-TEC GmbH

fluorescence

λex 680 nm; λem 695 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

2-8°C

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Application

Atto 680 has been used as a dye to study the fluorescence quantum yields of bioanalytically relevant red dyes.
Atto fluorescent labels are designed for high sensitivity applications, including single-molecule detection. Atto labels have rigid structures that do not show any cis-trans isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.
Atto 680 shows a molar extinction of 120,000 and QY of 30% in water (40% in ethanol). Decay time is 1.8 ns.

General description

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Other Notes

New red absorbing fluorescent dye as a biophysical probe for binding interactions; used as fluorescence tracer in spray diagnostics.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCD2363
BCBV6493
1343528
1338355
1331292

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Spray diagnostics using an all-solid-state Nd:YAIO3 laser and fluorescence tracers in commercial gasoline and diesel fuels.
I. Duwell et al.
Applied Physics B: Lasers and Optics, 79, 249-254 (2004)
Dual laser fluorescence correlation spectroscopy as a biophysical probe of binding interactions - evaluation of new red fluorescent dyes.
K.M. Swift et al.
Proc. SPIE: Int. Soc. Opt. Eng., 4252, 47-47 (2001)
Integrating sphere setup for the traceable measurement of absolute photoluminescence quantum yields in the near infrared
Wu?rth C, et al.
Analytical Chemistry, 84(3), 1345-1352 (2012)
Volker Buschmann et al.
Bioconjugate chemistry, 14(1), 195-204 (2003-01-16)
The spectroscopic characteristics (absorption, emission, and fluorescence lifetime) of 13 commercially available red-absorbing fluorescent dyes were studied under a variety of conditions. The dyes included in this study are Alexa647, ATTO655, ATTO680, Bodipy630/650, Cy5, Cy5.5, DiD, DY-630, DY-635, DY-640, DY-650
Irene Drude et al.
Biochemical and biophysical research communications, 363(1), 24-29 (2007-09-11)
Over the past two decades, the structure and mechanism of catalytic RNA have been extensively studied; now ribozymes are understood well enough to turn them into useful tools. After we have demonstrated the twin ribozyme mediated insertion of additional nucleotides
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荧光寿命测量

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