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83702

Sigma-Aldrich

Rhodamine 123

BioReagent, for fluorescence, ≥85% (HPLC)

Synonym(s):

2-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic acid methyl ester

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About This Item

Linear Formula:
C21H17ClN2O3
CAS Number:
62669-70-9
Molecular Weight:
380.82
Beilstein:
6030951
EC Number:
263-687-8
MDL number:
MFCD00012664
UNSPSC Code:
12352116
PubChem Substance ID:
57652966
NACRES:
NA.32

grade

for fluorescence

Quality Level

100

product line

BioReagent

Assay

≥85% (HPLC)

form

powder

impurities

≤10% water

solubility

ethanol: 20 mg/mL, clear, red

suitability

in accordance for fluorescence

SMILES string

Cl[H].COC(=O)c1ccccc1C2=C3C=CC(=N)C=C3Oc4cc(N)ccc24

InChI

1S/C21H16N2O3.ClH/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20;/h2-11,22H,23H2,1H3;1H

InChI key

MYFATKRONKHHQL-UHFFFAOYSA-N

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General description

Rhodamine 123 is a fluorescent dye.

Application

Rhodamine 123 has been used:
  • as a substrate for P-glycoprotein (P-gp) to read out functional transporter activity in fluorescent accumulation assays
  • to label the cells for estimating the P-gp expression by flow cytometry
  • in transport studies, was added to the apical to basolateral (AB-transport) or basolateral to apical (BA-transport)
  • the detection of mitochondrial membrane potential (ΔΨm)

Biochem/physiol Actions

Rhodamine 123 is usually used as a functional reporter for P-glycoprotein (P-gp) in flow cytometry.
Fluorescent dye most commonly used in flow cytometry as functional reporter for Pgp. Functional assays for MDR proteins are better prognostic indicators in cancer therapy than levels of MDR protein expression. Rh 123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA (7-amino-4-methylcoumarin-3-acetic acid). Recent reports indicate Rh 123 may also be a substrate of MRP1.

Other Notes

Supravital mitochondrial-specific dye

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Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCM6618
BCCL3983
BCCH9222
BCCG4466
BCCB7631

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What are the excitation and emission wavelengths for Rhodamine 123 (Product Nos. 83702 and R8004)?

    Using ethanol as the solvent, the excitation maximum is  511 nm and the emission maximum is 534 nm.  The recommended excitation and emission wavelengths in PBS are 488 and 515-575 nm, respectively.

  6. Why would one select Rhodamine 123 (Product Nos. 83702 and R8004) instead of other rhodamine compounds?

    Due to is comparatively low toxicity, Rhodamine 123 is the dye of choice for visualizing mitochondria or for cell viability measurements.

  7. What is the solubility of Rhodamine 123, (Product Nos. 83702 and R8004)?

    Rhodamine 123 is soluble in ethanol at 20 mg/mL, and has been reported soluble in ether, various fats and lipids, and has limited solubility in water (no concentration given).

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Experimental Parasitology, 134(4), 466-473 (2013)
L Benel et al.
Basic and applied histochemistry, 33(2), 71-80 (1989-01-01)
The use of the supravital mitochondrial-specific dye Rhodamine 123 (Rh 123) in combination with flow cytometry permits the monitoring of the changes in the mitochondrial transmembrane potential, reflecting the overall mitochondrial activity of the living cell. While this probe appears
X Ronot et al.
Biology of the cell, 57(1), 1-7 (1986-01-01)
Flow cytometry combines the advantages of microscopy and biochemical analysis in a single highly sensitive technique for a rapid examination of numerous individual living cells. It has become a potent and essential tool in the studies of the physiology of
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