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79454

Sigma-Aldrich

Ampliflu™ Red Western Blot Kit

for fluorescent detection

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UNSPSC Code:
12352200

usage

sufficient for 50 applications

fluorescence

λex 571 nm; λem 585 nm

storage temp.

2-8°C

General description

<p>find more information here</p>

Application

The Ampliflu Red Western Blot Kit provides an alternative to the classical chemiluminescence detection. When working with Ampliflu Red, standard protocols for the immunoblotting-procedure can be used until the detection step. No extra time for signal accumulation is necessary as it is for chemiluminescent signals. The use of Ampliflu Red is suitable for protein quantities between 1 ng and 1 μg per band of protein.

Principle

The kit contains the peroxidase substrate Ampliflu Red that is enzymatically converted to the highly fluorescent compound, resorufin. Fluorescent imaging is then performed on the immunoblot.

Analysis Note

Sensitivity: Limit of detection is 1 ng/band, but may vary depending on the antibodies used.

Legal Information

Ampliflu is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

Product No.
Description
  • Dimethyl sulfoxide, puriss. p.a., ACS reagent, ≥99.9% (GC) 3 mL

Kit Components Also Available Separately

Product No.
Description
SDS
  • 90101Ampliflu™ Red, for fluorescence, ≥98.0% (HPLC) 5 vial(s)SDS
  • 79382Phosphate buffered saline, tablet 5 tabletsSDS

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H302

Precautionary Statements

P264 - P270 - P301 + P312 - P501

Hazard Classifications

Acute Tox. 4 Oral

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. How should Product 79454, Ampliflu™ Red Western Blot Kit, be used?

    See the product information sheet for this product (under Documents, above).

  6. What is the stability of Product 79454, Ampliflu™ Red Western Blot Kit, after reconstitution?

    Upon dissolution in DMSO, the Ampliflu red should be stored at 4 deg C. in the dark. It is stable for 1 month. Re-use of the staining solution is not recommended.

  7.  How can background staining be reduced when using Product 79454, Ampliflu™ Red Western Blot Kit?

    An optimal signal to background ratio may be obtained by using a low fluorescence PVDF membrane and a 5% (w/v) BSA blocking solution.

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    Ask a Scientist here.

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