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71386

Sigma-Aldrich

Sodium chloride solution

BioUltra, for molecular biology, ~5 M in H2O

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Synonym(s):
Halite, NaCl, sodium chloride
Linear Formula:
NaCl
CAS Number:
7647-14-5
Molecular Weight:
58.44
Beilstein:
3534976
MDL number:
MFCD00003477
UNSPSC Code:
12352302
PubChem Substance ID:
57652296
NACRES:
NA.25

grade

for molecular biology

Quality Level

100

product line

BioUltra

form

liquid

concentration

~5 M in H2O

technique(s)

DNA extraction: suitable
LAMP: suitable

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

refractive index

n20/D 1.378

pH

5.0-7.0 (25 °C)

solubility

water: soluble

density

1.186 g/mL (5 M in water)

cation traces

Al: ≤1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.02
λ: 280 nm Amax: 0.01

suitability

suitable for molecular biology

application(s)

genomic analysis

SMILES string

[Na+].[Cl-]

InChI

1S/ClH.Na/h1H;/q;+1/p-1

InChI key

FAPWRFPIFSIZLT-UHFFFAOYSA-M

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General description

Sodium chloride is a vital nutrient that aids normal body functions in humans and animals. This chemical is commonly found in nature with a wide variety of biochemical applications, like intravenous fluids and density gradients. It is also used as a diluent to increase ionic strength in buffers or culture media. Sodium chloride is crucial for maintaining plasma osmolality and the extracellular fluid volume of the body. Excess NaCl leads to increased blood pressure, which has implications for cardiovascular and renal diseases. Sterile NaCl is a component of eye drop solutions. Traditionally, it has been used in high concentrations to preserve food since most bacteria cannot grow in high-salt conditions.

Application

Sodium chloride solution has been used as a component of:
  • polyethylene glycol (PEG) buffer for the purification of double-tagged products
  • NT2 buffer for the preparation of antibody-coated Protein A Sepharose (PAS) beads and immunoprecipitation of ribonucleoproteins (RNPs)
  • chilled lysis buffer for the isolation of single nuclei from mice kidney

Features and Benefits

  • Geologically stable
  • Suitable for purification, precipitation, and crystallization

Other Notes

For additional information on our range of Biochemicals, please complete this form.

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WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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