71376
Sodium chloride
BioUltra, for molecular biology, ≥99.5% (AT)
Synonym(s):
Salt, Halite, NaCl
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About This Item
Linear Formula:
NaCl
CAS Number:
Molecular Weight:
58.44
Beilstein:
3534976
EC Number:
MDL number:
UNSPSC Code:
12352302
PubChem Substance ID:
NACRES:
NA.25
grade:
Molecular Biology
for molecular biology
for molecular biology
form:
solid
solubility:
H2O: 1 M at 20 °C, clear, colorless
Recommended Products
grade
Molecular Biology
for molecular biology
vapor pressure
1.33 (1.00 mmHg) at 865 °C hPa
product line
BioUltra
Assay
≥99.5% (AT)
form
solid
availability
not available in (Sales restrictions may apply)
technique(s)
mass spectrometry (MS): suitable
western blot: suitable
impurities
DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
≤0.001% total nitrogen (N)
pH
5.0-8.0 (25 °C, 1 M in H2O)
mp
801 °C (lit.)
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General description
Sodium chloride, an essential nutrient, plays a critical role in biochemistry and molecular biology, making significant contributions to various biochemical applications. Within biological systems, it serves as a fundamental electrolyte, regulating osmotic pressure, pH balance, and electrical conductivity, all of which are essential for proper cellular function and vital physiological processes. Furthermore, sodium chloride plays a pivotal part in maintaining protein structure and promoting enzymatic activity, essential for stabilizing protein conformations and catalyzing biochemical reactions. Its role extends to the regulation of cellular homeostasis and the facilitation of ion and nutrient transport across cell membranes.It is an integral component of buffers like PBS and SSC, where it aids in various biochemical procedures, including the creation of density gradients and the fine-tuning of biological functions. Notably, sodium chloride is used to increase the ionic strength in buffers and culture media, impacting the ionic environment crucial for specific experiments and cell culture. Moreover, it is applied in DNA precipitation from samples containing SDS and the removal of small nucleic acid fragments during plasmid DNA preparations. Its utility extends to cell culture applications, where it plays a role in bacterial, yeast, and cell culture growth media.In molecular biology and cell culture, sodium chloride is an indispensable salt that finds its place in various aspects of research. It is a common constituent in cell culture media, lysis buffers, and protein storage solutions, contributing to the maintenance of optimal conditions for cell growth and experimentation. The sodium chloride used in these applications is of high purity, rigorously tested for impurities such as DNases, RNases, phosphatases, proteases, and trace metals, making it the ideal choice for molecular biology and biochemical research
Application
Sodium chloride has been used:
- as a component of buffer solution to prepare the pH solutions for open-circuit potential and cyclic voltammetry
- in the preparation of Bold’s basal medium (BBM)
- as a component of transcription and annealing buffer
- to maintain osmotic balance and ensure the purity and stability of the isolated extracellular vesicles (EVs)
- as a buffer for the lamination of lateral flow strips in the fabrication of lateral flow devices
Biochem/physiol Actions
Sodium chloride helps to stimulate the stable induction of T-helper cell 17 (TH17) cells.
Features and Benefits
- Suitable for molecular biology and biochecmial research
- Free from DNase, RNase, NICKase and protease
- Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications.
- Tested for the presence of Anionic and Cationic traces
Other Notes
For additional information on our range of Biochemicals, please complete this form.
This product is intended for research purposes only, and it is not meant for human consumption.
Component of lysis buffer and extraction buffer in the large-scale phenol extraction of RNA; Simple salting out procedure for extracting DNA from human nucleated cells
Sales restrictions may apply
comparable product
Product No.
Description
Pricing
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Axenic Cultivation of Mycelium of the Lichenized Fungus, Lobaria pulmonaria (Peltigerales, Ascomycota)
Cornejo C, et al.
Bio-protocol (2015)
Quantitative detection of C-reactive protein in human saliva using an electrochemical lateral flow device
Petruzzi et al.
Biosensors And Bioelectronics, 10, 100136-100136 (2022)
D.M. Wallace
Methods in Enzymology, 152, 39-39 (1987)
A solid-state thin-film Ag/AgCl reference electrode coated with graphene oxide and its use in a pH sensor
Kim T, et al.
Sensors, 15(3), 6469-6482 (2015)
Sodium chloride drives autoimmune disease by the induction of pathogenic T H 17 cells
Kleinewietfeld M, et al.
Nature, 496(7446), 518-518 (2013)
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