All Photos(1)
Synonym(s):
Imidazole solution, Glyoxaline solution
Empirical Formula (Hill Notation):
C3H4N2
CAS Number:
Molecular Weight:
68.08
Beilstein:
103853
MDL number:
UNSPSC Code:
12161700
PubChem Substance ID:
NACRES:
NA.25
Recommended Products
product line
BioUltra
Quality Level
form
liquid
concentration
1 M in H2O
impurities
DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
pH
9.5±0.5
density
1.01 g/mL at 20 °C
SMILES string
c1c[nH]cn1
InChI
1S/C3H4N2/c1-2-5-3-4-1/h1-3H,(H,4,5)
InChI key
RAXXELZNTBOGNW-UHFFFAOYSA-N
Related Categories
General description
Imidazole is used as a buffer in the pH range of 6.2-7.8 at 25°C. It is recommended as a component of a buffer for the assay of horseradish peroxidase and as a chelator for the binding of various divalent cations. Imidazole aids the elution of histidine-containing proteins from divalent cation resins (Sigma P6611, HisSelect.-HC Nickel affinity gel) and can also be used in reverse staining of SDS-PAGE gels for the detection of proteins.
Application
Imidazole buffer solution has been used as a component:
- of calmodulin-binding buffer 1 (CBB1) for the tandem affinity purification (TAP) of TAP-tagged proteins together with co-purifying proteins
- of lysis buffer, and SDS loading buffer for His-ubiquitin pull-down assays
- of imidazole wash and elution buffer for protein purification
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1 - Repr. 1B - Skin Corr. 1C
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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