53399
Anti-Rabbit IgG−Abberior® STAR 635P antibody produced in goat
for STED application
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Synonym(s):
Abberior® STAR 635P-Anti-Rabbit IgG antibody produced in goat
UNSPSC Code:
12352200
NACRES:
NA.45
Recommended Products
biological source
goat
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
rabbit
concentration
~1 mg/mL
fluorescence
λex 638 nm; λem 651 nm in PBS, pH 7.4
storage temp.
−20°C
General description
Abberior STAR 635P was the first member of an entirely new class of fluorescent dyes introduced exclusively by Abberior: the class of phosphorylated fluorescent dye. The dye excels in its photophysical parameters: it is very bright, very stable and it enables basically background free imaging. The dye works exceptionally well with Abberior Instruments STEDYCON und Expert line microscopes and Leica STED microscopes. Abberior STAR 635P can substitute Atto™ 647N, Alexa Fluor™ 647, or Cy®5. It can be excited with lasers at wavelength between 630 - 650 nm. For STED, a depletion wavelength around 750 - 800 nm is recommended. Abberior STAR 635P is the ideal partner for Abberior STAR 580 to obtain optimal 2 Color STED results. Best results are obtained with freshly prepared samples.
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 638 (PBS pH 7.4), 638 (H2O), 634 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: 120 000 (PBS pH 7.4), 120 000 (H2O), 130 000 (MeOH)
Correction Factor, CF260 = ε260/εmax: 0,21 (PBS pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0,40 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 651 (PBS pH 7.4), 651 (H2O), 651 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,90 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 3,3 (PBS pH 7.4)
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 638 (PBS pH 7.4), 638 (H2O), 634 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: 120 000 (PBS pH 7.4), 120 000 (H2O), 130 000 (MeOH)
Correction Factor, CF260 = ε260/εmax: 0,21 (PBS pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0,40 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 651 (PBS pH 7.4), 651 (H2O), 651 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,90 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 3,3 (PBS pH 7.4)
Suitability
Designed and tested for fluorescent super-resolution microscopy
Analysis Note
May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence
unconjugated dye ≤5% of total fluorescence
Other Notes
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Atto is a trademark of Atto-Tec GmbH
Cy is a registered trademark of Cytiva
abberior is a registered trademark of Abberior GmbH
related product
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Annals of neurology, 86(2), 316-321 (2019-05-31)
The pathogenesis of Yo-mediated paraneoplastic cerebellar degeneration (PCD) is unclear. We applied cerebrospinal fluid and serum from PCD patients as well as CDR2 and CDR2L antibodies to neuronal tissue, cancer cell lines, and cells transfected with recombinant CDR2 and CDR2L
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