45359
Epoxy Embedding Medium kit
embedding resin for electron microscopy
Synonym(s):
Epon™ substitute embedding medium kit
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About This Item
UNSPSC Code:
12171500
NACRES:
NA.47
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This Item | 810533 | 81053N | 810535 |
|---|---|---|---|
| assay ≥98.5% | assay ≥98% (purity) | assay ≥98% (purity) | assay ≥98% (purity) |
| technique(s) ELISA: suitable, western blot: suitable, Southern blotting: suitable | technique(s) blood typing: suitable ( ) | technique(s) blood typing: suitable ( ) | technique(s) blood typing: suitable ( ) |
| origin New Zealand origin | origin USA origin | origin USA origin | origin USA origin |
| biological source bovine | biological source bovine serum | biological source bovine serum | biological source bovine serum |
| Quality Level 100 | Quality Level 300 | Quality Level 300 | Quality Level 300 |
| form lyophilized | form lyophilized powder | form lyophilized powder | form lyophilized powder |
General description
The Epoxy Embedding Medium Kit is a very widely used embedding medium for electron microscopy. The embedding formulation originally published by Luft (1961) is excellent for both plant and animal tissues. Due to the low viscosity of the resin, it penetrates the tissue specimen faster than Araldite and other polymers. It hardens easily and uniformly at low temperatures with the addition of dodecenylsuccinic anhydride (DDSA), methyl nadic anhydride (MNA), and the accelerator 2,4,6-tris(dimethylaminomethyl)phenol (DMP-30). Slight shrinkage may occur during curing. This kit is useful for embedding a variety of tissues as a wide range of hardness can be obtained with this resin to suit a specific tissue type by using two different anhydride curing agents (DDSA and MNA).
Application
Epoxy Embedding Medium Kit has been used in the following applications:
- to study the role of NDRG2, an essential colonic epithelial barrier regulator, in gut homeostasis maintenance and colitis-associated tumor development[1]
- to study the interaction of polymer scaffold nanofibers with growing cells[2]
- to study the morphological characterization of postembryonic development of blood–spleen barrier in ducks[3]
- to understand the role of NOD1/NOD2 receptors in Fusobacterium nucleatum-mediated neutrophil extracellular traps[4]
Features and Benefits
- Excellent medium for embedding a wide variety of tissues, including both plant and animal.
- Facilitates rapid embedding (less than 3 hours).
- Low viscosity facilitates rapid tissue penetration.
- Desirable hardness is easy to obtain to suit a specific tissue type.
Components
45345 Epoxy Embedding Medium 2x250 ml
45347 Hardener MNA 250 ml
45346 Hardener DDSA 250 ml
45348 Accelerator DMP 30 250 ml
45347 Hardener MNA 250 ml
45346 Hardener DDSA 250 ml
45348 Accelerator DMP 30 250 ml
Legal Information
Epon is a trademark of Hexion, Inc.
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Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 4 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
8A - Combustible corrosive hazardous materials
Flash Point(F)
275.0 °F
Flash Point(C)
135 °C
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Role of NOD1/NOD2 receptors in Fusobacterium nucleatum mediated NETosis
Alyami, et al.
Microbial Pathogenesis (2019)
High resolution 3D microscopy study of cardiomyocytes on polymer scaffold nanofibers reveals formation of unusual sheathed structure
Balashov, et al.
Acta Biomaterialia (2018)
Investigation of the effects of semaphorin 3A on new bone formation in a rat calvarial defect model.
Sevinç Kenan et al.
Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery, 47(3), 473-483 (2019-01-09)
This study investigates the effects of semaphorin 3A on new bone formation in an experimental rat model. Cortical bone defects, 5 mm, were created in the calvaria of 40 Wistar rats, which were then separated into three groups: empty defect (control)
Kunio Nagashima et al.
Methods in molecular biology (Clifton, N.J.), 697, 83-91 (2010-12-01)
This chapter outlines the procedures for ex vivo TEM preparation of nanoparticle-containing tissue or cell culture samples using an epoxy resin embedding method. The purpose of this procedure is to preserve the structure of tissue in a hardened epoxy block
Arnold Lavaud et al.
Translational vision science & technology, 9(5), 13-13 (2020-08-22)
To evaluate visual streak (VS) identification on spectral-domain optical coherence tomography (SD-OCT) scans in awake rabbits. To report thickness measurements in the VS and adjacent retina on OCT B-scans and histologic sections and to assess inter-method bias, precision and repeatability
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