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Safety Information

43817

Sigma-Aldrich

DL-Dithiothreitol

BioUltra, ≥99.0% (RT)

Synonym(s):

threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT

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1 EA
CN¥6,780.43

CN¥6,780.43

Available to ship onApril 27, 2025Details

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About This Item

Linear Formula:
HSCH2CH(OH)CH(OH)CH2SH
CAS Number:
3483-12-3
Molecular Weight:
154.25
Beilstein:
1719757
EC Number:
222-468-7
MDL number:
MFCD00004877
UNSPSC Code:
12352200
PubChem Substance ID:
57650197

CN¥6,780.43

Available to ship onApril 27, 2025Details

Request a Bulk Order

product line

BioUltra

Assay

≥99.0% (RT)

form

solid

impurities

insoluble matter, passes filter test
≤0.3% 4,5-dihydroxy-1,2-dithiane

pH

4.0-6.5 (25 °C, 0.1 M in H2O)

mp

41-44 °C (lit.)

solubility

H2O: 0.1 M at 20 °C, clear, colorless

anion traces

sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.5 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤100 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

0.1 M in H2O

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43815D554543819
DL-Dithiothreitol BioUltra, ≥99.0% (RT)

43817

DL-Dithiothreitol

DL-Dithiothreitol BioUltra, Molecular Biology, ≥99.5% (RT)

43815

DL-Dithiothreitol

DL-Dithiothreitol BioXtra, ≥99.0% (titration)

D5545

DL-Dithiothreitol

DL-Dithiothreitol ≥99.0% (RT)

43819

DL-Dithiothreitol

assay

≥99.0% (RT)

assay

≥99.5% (RT)

assay

≥99.0% (titration)

assay

≥99.0% (RT)

product line

BioUltra

product line

BioUltra

product line

BioXtra

product line

-

impurities

insoluble matter, passes filter test, ≤0.3% 4,5-dihydroxy-1,2-dithiane

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤0.3% 4,5-dihydroxy-1,2-dithiane

impurities

Insoluble matter, passes filter test, ≤0.3% 4,5-dihydroxy-1,2-dithiane

impurities

≤0.3% 4,5-dihydroxy-1,2-dithiane

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

form

solid

form

powder

form

powder

form

powder

anion traces

sulfate (SO42-): ≤50 mg/kg

anion traces

sulfate (SO42-): ≤50 mg/kg

anion traces

sulfate (SO42-): ≤0.005%

anion traces

sulfate (SO42-): ≤50 mg/kg

Application

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

Other Notes

Cleavage of disulfide bonds in proteins;[1] In the investigation of incremental and kinetic reduction in proteins;[2] Prevents the oxidation of cholecystokinin during its extraction from brain and duodenal tissues;[3] In protein sequence analysis.[4]

Pictograms

CorrosionExclamation mark
GHS05,GHS07

Signal Word

Danger

Hazard Statements

H302,H315,H318

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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    D W Sears et al.
    Biochemistry, 16(9), 2031-2035 (1977-05-03)
    The reduction by dithiothreitol (DTT) of the four interchain disulfides of a human IgGlkappa immunoglobulin has been studied by two methods: variation of the concentration of DTT relative to the protein concentration (incremental reduction); and variation of the time of
    J R Jabusch et al.
    Analytical biochemistry, 91(2), 532-542 (1978-12-01)
    The thiazolinone and phenylthiohydantoin derivatives of most amino acids can be hydrolyzed with alkaline dithionite to generate the free amino acid. The acidification of this hydrolysate with 3 N HCl containing thiodiglycol leads in the case of glutamic acid, glutamine
    A.Bacarese-Hamilton et al.
    Annals of the New York Academy of Sciences, 448, 571-571 (1985)
    Reductive cleavage of cystine disulfides with tributylphosphine.
    U T Rüegg et al.
    Methods in enzymology, 47, 111-116 (1977-01-01)
    Xueqian Liu et al.
    The Journal of biological chemistry, 288(10), 7230-7240 (2013-01-25)
    In Pichia pastoris, the peroxisomal targeting signal 2 (PTS2)-dependent peroxisomal matrix protein import pathway requires the receptor, Pex7, and its co-receptor Pex20. A conserved lysine (Lys(19)) near the N terminus of Pex20 is required for its polyubiquitination and proteasomal degradation
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