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Key Documents

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Safety Information

32884

Sigma-Aldrich

Giemsa Stain

Modified Solution

Synonym(s):

Azure eosin methylene blue, Giemsa solution

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About This Item

CAS Number:
51811-82-6
MDL number:
MFCD00081642
UNSPSC Code:
12171500
PubChem Substance ID:
329754377
NACRES:
MA.02
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Product Name

Giemsa Stain, Modified Solution, according to Giemsa

form

liquid

Quality Level

200

quality

according to Giemsa

technique(s)

UV/Vis spectroscopy: suitable

color

dark blue

pH

6.9

density

1.036 g/cm3 at 20 °C

suitability

passes test for UV spectrum

application(s)

diagnostic assay manufacturing
hematology
histology

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Show Differences

1 of 4

This Item
497283067442424
Sigma-Aldrich

92716

Atto 620

Sigma-Aldrich

49728

Atto 620 maleimide

Atto 700 BioReagent, suitable for fluorescence, ≥90.0% (HPCE)

30674

Atto 700

Sigma-Aldrich

42424

Atto 550

fluorescence

λex 617 nm; λem 641 nm in 0.1 M phosphate pH 7.0

fluorescence

λex 619 nm; λem 643 nm in 0.1 M phosphate pH 7.0

fluorescence

λex 681 nm; λem 714 nm in 0.1 M phosphate pH 7.0

fluorescence

-

form

powder

form

solid

form

-

form

powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

assay

≥90% (HPCE)

assay

≥80% (coupling to thiols)

assay

≥90.0% (HPCE)

assay

≥90% (HPLC)

product line

BioReagent

product line

-

product line

BioReagent

product line

-

General description

Modified Giemsa stain means when the pH of the Giemsa stain is changed from the usual 6.8 to 9.0.[1]

Application

Modified Giemsa stain may be used for the identification of each human chromosome by differentials staining.[1]

Other Notes

Giemsa stain, modified, 0.4%, w/v, in buffered methanol solution, pH 6.8. Also contains stabilizers.

Principle

When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration. The use of purified eosin and thiazine dyes minimizes lot-to-lot variation.

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1

Target Organs

Eyes,Central nervous system

Storage Class Code

3 - Flammable liquids

WGK

WGK 2

Flash Point(F)

51.8 °F - closed cup

Flash Point(C)

11 °C - closed cup

Regulatory Information

危险化学品
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    BCCM6963
    BCCL3477
    BCCM2102
    BCCJ7601
    BCCK3962

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    S R Patil et al.
    Science (New York, N.Y.), 173(3999), 821-822 (1971-08-27)
    Differential staining of human chromosomes can be obtained when the pH of Giemsa stain is changed to 9.0 from the usual 6.8. Such staining permits identification of all homolog pairs and distinct regions within chromosome arms. In most instances, the
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