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Merck
CN

21879

5(6)-Carboxyfluorescein diacetate

BioReagent, suitable for fluorescence, ≥90.0% (HPLC)

Synonym(s):

5(6)-Carboxy-di-O-acetylfluorescein

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About This Item

Empirical Formula (Hill Notation):
C25H16O9
CAS Number:
124387-19-5
Molecular Weight:
460.39
NACRES:
NA.32
PubChem Substance ID:
329752214
UNSPSC Code:
12352108
MDL number:
MFCD00467494

Key Documents

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Product Name

5(6)-Carboxyfluorescein diacetate, BioReagent, suitable for fluorescence, ≥90.0% (HPLC)

InChI

1S/2C25H16O9/c1-12(26)31-15-4-7-18-21(10-15)33-22-11-16(32-13(2)27)5-8-19(22)25(18)20-9-14(23(28)29)3-6-17(20)24(30)34-25;1-12(26)31-14-6-8-18-20(10-14)33-21-11-15(32-13(2)27)7-9-19(21)25(18)22-16(23(28)29)4-3-5-17(22)24(30)34-25/h2*3-11H,1-2H3,(H,28,29)

SMILES string

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5ccc(cc45)C(O)=O)c1.CC(=O)Oc6ccc7c(Oc8cc(OC(C)=O)ccc8C79OC(=O)c%10cccc(C(O)=O)c9%10)c6

InChI key

SWRGCNMDGRMQGB-UHFFFAOYSA-N

product line

BioReagent

assay

≥90.0% (HPLC)

form

powder

solubility

DMSO: soluble

fluorescence

λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (esterase)

suitability

suitable for fluorescence

storage temp.

2-8°C

Quality Level

100

Related Categories

Application

5(6)-Carboxyfluorescein diacetate (CFDA) is a dye which is been extensively utilized to label cultured neurons with the essential requirements, in a specific manner.

5(6)- Carboxyfluorescein diacetate (CFDA) is a viability probe that assesses enzymatic activity, which is necessary to activate its fluorescence, and cell-membrane integrity, which is necessary for the retention of their fluorescent product within the cell.

General description

5(6)-Carboxyfluorescein diacetate or CDFDA is a fluorescent oxidant sensing probe. It is an intracellular probe added to the cells as a pro-substrate, which upon hydrolysis by the esterase enzyme produces the fluorescent product called CDF.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCD6495
BCCD4531
BCBT5849
BCBP1269V
BCBN5958V

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Ping Li et al.
The New phytologist, 215(2), 699-710 (2017-04-07)
The interactions of vector-virus-plant have important ecological and evolutionary implications. While the tripartite interactions have received some attention, little is known about whether vector infestation affects subsequent viral transmission and infection. Working with the whitefly Bemisia tabaci, begomovirus and tobacco/tomato
Tsui-Chun Hsu et al.
PloS one, 13(4), e0194866-e0194866 (2018-04-12)
Zearalenone (ZEN) is a mycotoxin produced by Fusarium species, which is one of the main animal feed contaminants causing reproductive disorders in livestock. The aim of this study was to evaluate the probiotic characteristics and ZEN removal ability of a
Rodrigo Anibal Mateos-Nava et al.
Drug and chemical toxicology, 40(4), 457-462 (2016-12-13)
Vanadium is a widely distributed metal in the Earth's surface and is released into the environment by either natural or anthropogenic causes. Vanadium (III) oxide (V
R E Petroski et al.
Journal of neuroscience methods, 52(1), 23-32 (1994-04-01)
A method for selectively labeling cultured neurons using the vital dye, 5(6)-carboxyfluorescein diacetate (CFDA), is described. This non-fluorescent membrane-permeant dye is cleaved by cytosolic esterases into the fluorescent anion, 5(6)-carboxyfluorescein (CF). Both astrocytes and neurons exhibit brilliant fluorochromasia within minutes
Zhiyuan Zhang et al.
The Plant cell, 29(8), 2027-2046 (2017-07-28)
Plasmodesmata (PDs) play vital roles in cell-to-cell communication and plant development. Emerging evidence suggests that sterols are involved in PD activity during cytokinesis. However, whether sterols contribute to PD gating between established cells remains unknown. Here, we isolated
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