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17195

Sigma-Aldrich

Fura 2 pentapotassium salt

for fluorescence, ≥90% (HPLC)

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Empirical Formula (Hill Notation):
C29H22K5N3O14
CAS Number:
113694-64-7
Molecular Weight:
831.99
MDL number:
MFCD00083328
UNSPSC Code:
12171500
PubChem Substance ID:
57647616
NACRES:
NA.32

grade

for fluorescence

Quality Level

100

Assay

≥90% (HPLC)

form

solid

solubility

H2O: soluble

fluorescence

λex 355 nm; λem 495 nm in 0.1 M Tris pH 8.0, Ca2+

storage temp.

−20°C

SMILES string

[K+].[K+].[K+].[K+].[K+].Cc1ccc(N(CC([O-])=O)CC([O-])=O)c(OCCOc2cc3cc(oc3cc2N(CC([O-])=O)CC([O-])=O)-c4ncc(o4)C([O-])=O)c1

InChI

1S/C29H27N3O14.5K/c1-15-2-3-17(31(11-24(33)34)12-25(35)36)20(6-15)43-4-5-44-21-7-16-8-22(28-30-10-23(46-28)29(41)42)45-19(16)9-18(21)32(13-26(37)38)14-27(39)40;;;;;/h2-3,6-10H,4-5,11-14H2,1H3,(H,33,34)(H,35,36)(H,37,38)(H,39,40)(H,41,42);;;;;/q;5*+1/p-5

InChI key

HQKWBBCTCLUGSX-UHFFFAOYSA-I

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Other Notes

A very sensitive fluorescent calcium chelator that exhibits a spectral shift in excitation maxima on binding calcium

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Measurement of Ca2+ release in skinned fibers from skeletal muscle.
M Endo et al.
Methods in enzymology, 157, 12-26 (1988-01-01)
G Grynkiewicz et al.
The Journal of biological chemistry, 260(6), 3440-3450 (1985-03-25)
A new family of highly fluorescent indicators has been synthesized for biochemical studies of the physiological role of cytosolic free Ca2+. The compounds combine an 8-coordinate tetracarboxylate chelating site with stilbene chromophores. Incorporation of the ethylenic linkage of the stilbene

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