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Merck
CN

M0178

Middlebrook 7H9 Broth Base

NutriSelect® Plus, powder, for mycobacteria, pkg of 500 g

Synonym(s):

Mycobacterial culture media

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About This Item

NACRES:
NA.85
UNSPSC Code:
41171607

Key Documents

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Product Name

Middlebrook 7H9 Broth Base, NutriSelect® Plus, powder, for mycobacteria, pkg of 500 g

sterility

non-sterile

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

packaging

pkg of 500 g

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbe id | susceptibility testing: suitable
microbiological culture: suitable

final pH

6.6±0.2 (25 °C)

application(s)

clinical testing
food and beverages
life science and biopharma
microbiology
veterinary

storage temp.

2-25°C

suitability

mycobacteria

Quality Level

200

Related Categories

Application

Middlebrook 7H9 Broth Base has been used for the cultivation Mycobacterium bovis bacillus Calmette-Guérin Pasteur strain. It is also recommended for cultivation and sensitivity testing of Mycobacterium tuberculosis.

General description

Middlebrook 7H9 Broth Base contains inorganic compounds that supply the elements potassium, sulfur, magnesium, and phosphorus which are required for appropriate growth of Mycobacteria. This medium with Middlebrook ADC Growth Supplement is recommended for ideal growth of Mycobacterium species.
Middlebrook ADC Growth Supplement (Product No. M0553) contains bovine albumin, dextrose, and catalase. Dextrose supplies energy, while albumin protects Mycobacteria from toxic agents and catalase splits toxic radicals.
Mycobacteria grow most rapidly in broth media and hence primary isolation of bacterial species can be carried out in Middlebrook 7H9 Broth Base.

Other Notes

Ingredients (g/L)
Ammonium sulfate, 0.50
Disodium phosphate, 2.50
Monopotassium phosphate, 1.00
Sodium citrate, 0.10
Magnesium sulfate, 0.05
Calcium chloride, 0.0005
Zinc sulfate, 0.001
Copper sulfate, 0.001
Ferric ammonium citrate, 0.04
L-Glutamic acid, 0.50
Pyridoxine, 0.001
Biotin, 0.0005
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Preparation Note

Suspend 2.35 g of Middlebrook 7H9 Broth Base in 450 ml of distilled water. Add either 2 ml of glycerol (Cat. No 49767) or 0.5 g of TWEEN® 80 (Cat. No. 93780). Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. Pressure (121°C) for 10 minutes. Cool to 45°C or below and aseptically add 1 vial of Middlebrook ADC Growth Supplement (Cat. No. M0553). Mix well before dispensing.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

Storage Class

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

动植物来源培养基
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Certificates of Analysis (COA)

Lot/Batch Number
BCCN2053
BCCL9635
BCCK4930
BCCH9535
BCCG5969

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Peter Masoko et al.
Evidence-based complementary and alternative medicine : eCAM, 2013, 586247-586247 (2013-08-21)
The aim of the study was to scientifically evaluate the antimycobacterial activity of selected indigenous medicinal plants from the Limpopo Province used for the treatment of humans with symptoms of Mycobacterium tuberculosis. The leaves of five plant species (Apodytes dimidiata
Differential requirements for L-citrulline and L-arginine during antimycobacterial macrophage activity
Rapovy SM, et al.
Journal of Immunology, 195(7), 3293-3300 (2015)
l-Citrulline Metabolism in Mice Augments CD4+ T Cell Proliferation and Cytokine Production In Vitro, and Accumulation in the Mycobacteria-Infected Lung
Lange S M, et al.
Frontiers in Immunology, 8, 1561-1561 (2017)
Nancy Patience Motlalepula Komape et al.
BMC complementary and alternative medicine, 17(1), 128-128 (2017-02-27)
Tuberculosis is an infectious communicable disease and the causative agent of the disease has over the years developed resistance to streamline chemotherapeutic agents with dire consequences and there is a need for development of new and more potent alternatives. Constituents
Daniel J Greenwood et al.
Science (New York, N.Y.), 364(6447), 1279-1282 (2019-06-30)
Tuberculosis, caused by the intracellular pathogen Mycobacterium tuberculosis, remains the world's deadliest infectious disease. Sterilizing chemotherapy requires at least 6 months of multidrug therapy. Difficulty visualizing the subcellular localization of antibiotics in infected host cells means that it is unclear
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Detection & Molecular Identification of Mycobacteria

Identifying Mycobacterium species crucial due to varying clinical significance; some pathogenic, others not.

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