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H3136

Sigma-Aldrich

Hematoxylin

certified by the Biological Stain Commission

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Synonym(s):
Natural Black 1
Empirical Formula (Hill Notation):
C16H14O6 · xH2O
CAS Number:
517-28-2
Molecular Weight:
302.28 (anhydrous basis)
Colour Index Number:
75290
Beilstein:
91400
EC Number:
208-237-3
MDL number:
MFCD00078111
UNSPSC Code:
12171500
PubChem Substance ID:
24895554
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

200

form

powder

mp

200 °C (dec.) (lit.)

solubility

ethanol: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Oc1cc2C[C@@]3(O)COc4c(O)c(O)ccc4[C@H]3c2cc1O

InChI

1S/C16H14O6/c17-10-2-1-8-13-9-4-12(19)11(18)3-7(9)5-16(13,21)6-22-15(8)14(10)20/h1-4,13,17-21H,5-6H2/t13-,16+/m0/s1

InChI key

WZUVPPKBWHMQCE-XJKSGUPXSA-N

Gene Information

rat ... Adora1(29290) , Adora2a(25369)

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General description

Hematoxylin is a basophilic dye extracted from the tree Heamatoxylum campechianum. It specifically binds to acidic compounds and thus is useful for staining cell nuclei which contain acidic substances: DNA and RNA. Hematoxylin, when oxidized forms hematein, which has thick pale blue purple colour. Hematoxylin is often used along with eosin, in histological study under light microscope.

Application

Hematoxylin has been used:
  • to visualize anatomical features and to detect nucleus locus coeruleus adjacent to the periaqueductal gray region in brainstem samples
  • as a nuclear counterstain to visualize the nucleus, adipocytes were counterstained with a hematoxylin reagent
  • to stain lung tissue for histological analysis

Suitability

Certified for use in Weigert′s iron hematoxylin, Mayer′s hemalum, Harris hemalum and in Heidenhain′s technique

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319

Hazard Classifications

Eye Irrit. 2

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Expression of matrilin-2 in liver cirrhosis and hepatocellular carcinoma
Szabo E, et al.
Pathology Oncology Research, 14(1), 15-22 (2008)
Histologic validation of locus coeruleus MRI contrast in post-mortem tissue.
Keren NI
Neuroimage, 113, 235-235 (2015)
Techniques in Aquatic Toxicology (2005)
Expression of matrilin-2 in liver cirrhosis and hepatocellular carcinoma.
Szabo E
Pathology Oncology Research, 14, 15-15 (2008)
S-nitrosylation of fatty acid synthase regulates its activity through dimerization.
Choi MS
Journal of Lipid Research, 57, 607-607 (2016)
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