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Safety Information

F6797

Millipore

Frey mycoplasma broth base

suitable for microbiology, NutriSelect® Plus

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UNSPSC Code:
41171607
NACRES:
NA.85

sterility

non-sterile

Quality Level

200

form

powder

shelf life

limited shelf life, expiry date on the label

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.7±0.2 (25 °C)

application(s)

clinical testing
food and beverages
life science and biopharma
veterinary
microbiology

suitability

mycoplasma

Related Categories

Application

Used for cultivation of Avian Mycoplasma.
Frey mycoplasma broth base may be used as a culture medium for examining various proteins of Mycoplasma pulmonis.

Components

Ingredients (g/L)
Casein enzymic hydrolysate, 7.50
Papaic digest of soyabean meal, 2.50
Yeast extract, 5.00
Sodium chloride, 5.00
Potassium chloride, 0.40
Magnesium sulphate, 0.20
Disodium phosphate, 1.60
Monopotassium phosphate, 0.10

Preparation Note

Suspend 22.3 g of Frey Mycoplasma Broth Base in 900 ml of distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add 100 ml of sterile heat inactivated horse serum (H1138). Mix well before dispensing.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

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Protein variability among strains of Mycoplasma pulmonis.
Watson HL, et al.
Infection and Immunity, 55(11), 2838-2840 (1987)

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