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E8751

Sigma-Aldrich

Ethidium bromide

~95% (HPLC)

Synonym(s):

3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide, EtBr, Homidium bromide

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50 G
CN¥545.27

CN¥545.27

List PriceCN¥1,817.57Save 70%

Available to ship onApril 27, 2025Details

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50 G
CN¥545.27

About This Item

Empirical Formula (Hill Notation):
C21H20BrN3
CAS Number:
1239-45-8
Molecular Weight:
394.31
Beilstein:
3642536
EC Number:
214-984-6
MDL number:
MFCD00011724
UNSPSC Code:
41105322
PubChem Substance ID:
24894683
NACRES:
NB.22

CN¥545.27

List PriceCN¥1,817.57Save 70%

Available to ship onApril 27, 2025Details

Request a Bulk Order

Assay

~95% (HPLC)

Quality Level

200

mp

260-262 °C (dec.) (lit.)

SMILES string

[Br-].CC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14

InChI

1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H

InChI key

ZMMJGEGLRURXTF-UHFFFAOYSA-N

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Show Differences

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21223391947900195
碱式磷酸钙 powder, 5 μm

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碱式磷酸钙

羟基磷灰石 purum p.a., ≥90% (as Ca3(PO4)2, KT)

21223

羟基磷灰石

Hydroxylapatite, Fast Flow

391947

Hydroxylapatite, Fast Flow

碱式磷酸钙 powder, 2.5 μm

900195

碱式磷酸钙

form

powder

form

powder

form

solid

form

powder

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

description

Particle size: <5.0 μm ± 1.0 μm

description

-

description

-

description

Particle size: <2.5 μm± 0.5 μm, Total Heavy Metals: ≤20 ppm

surface area

≥80 m2/g

surface area

-

surface area

-

surface area

≥80 m2/g

particle size

5 μm

particle size

-

particle size

-

particle size

-

Application

Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.

Frameshift mutagen which intercalates double-stranded DNA and RNA.

Biochem/physiol Actions

Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.

Reconstitution

For staining a gel after electrophoresis, dilute a sample of the stock solution to 0.5 μg/ml with water and incubate the gel for 15-30 min. Destaining is usually not needed but can be carried out in water for 15 min if decreased background is necessary. The DNA bands can then be detected on a UV light box (254 nm wavelength). Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 μg/ml and visualized immediately after electrophoresis.

related product

Product No.
Description
Pricing

Pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

Signal Word

Danger

Hazard Statements

H302,H330,H341

Hazard Classifications

Acute Tox. 1 Inhalation - Acute Tox. 4 Oral - Muta. 2

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves
  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    SHBR4830
    SHBQ7920
    SHBQ5224
    MKCM8424
    0000104516

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    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

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    1. Which document(s) contains shelf-life or expiration date information for a given product?

      If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

    2. How do I get lot-specific information or a Certificate of Analysis?

      The lot specific COA document can be found by entering the lot number above under the "Documents" section.

    3. How do I find price and availability?

      There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

    4. What is the best working concentration of Product E8751, Ethidium bromide?

      For staining a gel after electrophoresis, dilute a sample of the stock solution to 0.5 μg/mL with water and incubate the gel for 15-30 min.  Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 μg/mL, and visualized immediately after electrophoresis.

    5. How can I make a stock solution of Product E8751, Ethidium bromide?

      Ethidium bromide is soluble in water at 100 mg/mL. Per Crary, B. and Boyrsenko, M., Biotechniques, 4, 98-101 (1986), stock solutions of ethidium bromide in water or PBS are stable for at least two years at room temperature if protected from light.

    6. How can I dispose of solutions containing Product E8751, Ethidium bromide?

      Ethidium bromide is mutagenic, and ultimately has to be destroyed by incineration. It can be removed from buffer solutions by several methods to reduce the volume of waste. See the product sheet for more details.

    7. What is the Department of Transportation shipping information for this product?

      Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

    8. My question is not addressed here, how can I contact Technical Service for assistance?

      Ask a Scientist here.

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