Quality Level
Assay
≥95.0% (HPCE)
form
liquid
greener alternative product characteristics
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Greener Alternative Product
solubility
DMSO: soluble
fluorescence
λex 520 nm; λem 554 nm in TE buffer; DNA
greener alternative category
storage temp.
2-8°C
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General description
Application
In addition, Nancy-520 can be used to determine dsDNA concentrations in solution, with a linear range between 0 - 2 μg/ml of DNA. Nancy 520
Nancy-520 has been used successfully in PCR (Real time-PCR) applications.
Nancy 520
Greener alternative to Ethidium bromide.
Packaging
related product
WGK
WGK 2
Flash Point(F)
closed cup
Flash Point(C)
closed cup
Personal Protective Equipment
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the mutagenic potential of Product 01494, Nancy-520, compared to SYBR Green I and ethidium bromide?
The Ames test (II) has confirmed that Product 01494, Nancy-520, is less mutagenic than SYBR green I and ethidium bromide.
What is the sensitivity of Product 01494, Nancy-520, compared to SYBR Green I and ethidium bromide?
Product 01494, Nancy-520, shows a higher sensitivity than ethidium bromide and a slightly higher sensitivity to SYBR Green I.
Does Product 01494, Nancy-520, require the same safety handling that is required for Ethidium bromide?
No, the handling of Product 01494, Nancy-520, does not require the strict safety instructions that are needed for Ethidium bromide. Normal latex gloved may be used. No specific "Nitrile" gloves are required. No particularly equipped fume hood for Nancy-520 is required. Other work can be done in the same fume hood, where Nancy-520 is used.
Can Product 01494, Nancy-520 staining solution, be reused?
Re-use of Product 01494, Nancy-520 staining solution, is not recommended.
Can Nancy-520 be detected when using the Odyssey® Fc imager?
According to an application note published by LI-COR "Imaging Nucleic Acid Gels on the Odyssey® Fc Imager", published in 2011, Nancy-520 was successfully tested in the Fc imager, using the 600 channel.®Odyssey is a registered Trademark of LI-COR, Inc. The trademark belongs to their respective owners. The Odyssey® Fc Imaging system is covered by U.S. patents, foreign equivalents, and patents pending.
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Articles
Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR
Related Content
Nancy-520 for DNA Detection and Quantitation
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