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E4382

Sigma-Aldrich

Eosin Y disodium salt

Dye content, ≥90%, certified by the Biological Stain Commission, powder

Synonym(s):

2′,4′,5′,7′-Tetrabromofluorescein disodium salt, Acid Red 87, Bromo acid J. TS, XL, or XX, Bromofluorescein, Bronze Bromo ES, D&C;Red No. 22, Eosin yellowish

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About This Item

Empirical Formula (Hill Notation):
C20H6Br4Na2O5
CAS Number:
17372-87-1
Molecular Weight:
691.85
Colour Index Number:
45380
Beilstein:
3586809
EC Number:
241-409-6
MDL number:
MFCD00005040
UNSPSC Code:
12171500
PubChem Substance ID:
24894551
NACRES:
NA.47

Product Name

Eosin Y disodium salt, certified by the BSC

Agency

certified by the BSC

Quality Level

200

form

powder

composition

Dye content, ≥90%

technique(s)

titration: suitable

color

brown

pH

6.55 (29 °C, 84 °F)

mp

255 — 270 °C (491 - 518 °F at 977.9 hPa)

solubility

H2O: 1 mg/mL

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胎牛血清 non-USA origin, γ-irradiated, sterile-filtered, suitable for cell culture

F0679

胎牛血清

胎牛血清 USA origin, suitable for cell culture

12103C

胎牛血清

origin

USA origin

origin

New Zealand origin

origin

non-USA origin

origin

USA origin

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

sterility

sterile-filtered

sterility

sterile-filtered

sterility

γ-irradiated, sterile-filtered

sterility

-

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

impurities

≤25 EU/mL endotoxin

impurities

≤10 EU/mL endotoxin

impurities

≤10 EU/mL endotoxin

impurities

≤10 EU/mL endotoxin

Application

Eosin Y disodium salt has been used as a counterstain in immunocytochemistry and immunohistochemistry.[1][2]

Biochem/physiol Actions

Eosin Y is a xanthene dye and is used for the differential staining of connective tissue and cytoplasm. In histopathology, it is applied as a counterstain after hematoxylin and before methylene blue. It is also used as a background stain, thereby giving contrast to the nuclear stains.[3][4]

Suitability

Certified for use as a counterstain against hematoxylin; as a constituent of Wright stain; in modified Nocht′s stain; as a constituent in eosin-methylene blue medium.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317,H319

Hazard Classifications

Eye Irrit. 2 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

376.9 °F - Pensky-Martens closed cup

Flash Point(C)

191.6 °C - Pensky-Martens closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves
  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000421986
    0000421986
    SHBS3896
    0000410611
    0000368238

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    Dendritic cell loss from nonlymphoid tissues after systemic administration of lipopolysaccharide, tumor necrosis factor, and interleukin 1.
    Roake JA, et al.
    The Journal of Experimental Medicine, 181, 2237-2237 (1995)
    Different expression of occludin and ZO-1 in primary and metastatic liver tumors.
    Orban E, et al.
    Pathology Oncology Research, 14, 299-299 (2008)
    Bancroft JD
    Theory and Practice of Histological Techniques (2008)
    Joanne M Donkers et al.
    Cellular and molecular gastroenterology and hepatology, 10(3), 451-466 (2020-04-25)
    Bile acids are important metabolic signaling molecules. Bile acid receptor activation promotes body weight loss and improves glycemic control. The incretin hormone GLP-1 and thyroid hormone activation of T4 to T3 have been suggested as important contributors. Here, we identify
    Histophatologic Techniques null
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